| Citation | Li L, Tang X, Guo X, Rao D, Zeng L, Xue J, Liu S, Tu S, Shen EZ. Spatiotemporal single-cell architecture of gene expression in the Caenorhabditis elegans germ cells Cell Discov, 2025. |
| PubMed ID | 40097379 |
| Short Description | Spatiotemporal single-cell architecture of gene expression in the Caenorhabditis elegans germ cells GEO Record: GSE274290, Platform: GPL26672 Lab Atlas: https://scgerm-atlas.sjtu.edu.cn/website/#/home Other resources: N.A. |
| # of Conditions | 1 |
Full Description
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Spermatogenesis is an intricate and tightly controlled process, encompassing various layers of gene expression regulation. Despite our current understanding, the developmental trajectory and its regulatory mechanisms dictating spermatogenesis remain elusive. In this study, we have generated single-cell gene expression profiles for C. elegans sperm cells and constructed gene-regulated networks alongside the developmental trajectories of these cells. Our findings indicate that each pre- and post-developmental stage is closely linked by co-expressed genes, while simultaneously being uniquely identified by the combined expression of specific gene families. To illustrate the applicability of this exhaustive gene expression catalog, we used gene-regulated networks to uncover potential transcription factors for (1) the expression of genes in the phosphorylation pathway, identifying NHR-23-to-phosphatase regulation for the meiotic cell division process, and (2) the expression of constituent components of small RNA pathways, identifying ELT-1-to-Argonaute protein regulation for siRNA maintenance and sperm activation. We expect that this sperm cell-specific gene expression directory will prompt investigations into the underlying mechanisms determining anatomy, differentiation, and function across the reproductive system. Finally, our expression data can be explored using the web application CelegansGermAtlas (https://scgerm-atlas.sjtu.edu.cn/website/#/home). |
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