SPELL - Nematode

Citation	Bagijn MP, Goldstein LD, Sapetschnig A, Weick EM, Bouasker S, Lehrbach NJ, Simard MJ, Miska EA. Function, targets, and evolution of Caenorhabditis elegans piRNAs. Science, 2012.
PubMed ID	22700655
Short Description	Function, targets, and evolution of Caenorhabditis elegans piRNAs. GEO Record: GSE37432 GSE37433 Platform: GPL200 Download gene-centric, log2 transformed data: WBPaper00041191.ce.mr.csv
# of Conditions	9
Full Description	Piwi-interacting RNAs (piRNAs) are small RNAs required to maintain germline integrity and fertility, but their mechanism of action is poorly understood. Here we demonstrate that Caenorhabditis elegans piRNAs silence transcripts in trans through imperfectly complementary sites. Target silencing is independent of Piwi endonuclease activity or "slicing." Instead, piRNAs initiate a localized secondary endogenous small interfering RNA (endo-siRNA) response. Endogenous protein-coding gene and transposon transcripts exhibit Piwi-dependent endo-siRNAs at sites complementary to piRNAs and are derepressed in Piwi mutants. Genomic loci of piRNA biogenesis are depleted of protein-coding genes and tend to overlap the start and end of transposons in sense and antisense, respectively. Our data suggest that nematode piRNA clusters are evolving to generate piRNAs against active mobile elements. Thus, piRNAs provide heritable, sequence-specific triggers for RNA interference in C. elegans. Experimental Details: WBPaper00041191:N2_rep1 WBPaper00041191:N2_rep2 WBPaper00041191:N2_rep3 WBPaper00041191:piwi_n4357_n4358_rep1 WBPaper00041191:piwi_n4357_n4358_rep2 WBPaper00041191:piwi_n4357_n4358_rep3 WBPaper00041191:piwi_n4503_nDf57_rep1 WBPaper00041191:piwi_n4503_nDf57_rep2 WBPaper00041191:piwi_n4503_nDf57_rep3.
Tags	Method: microarray, Species: Caenorhabditis elegans, Topic: WT vs. mutant