| Citation | Kouns NA, Nakielna J, Behensky F, Krause MW, Kostrouch Z, Kostrouchova M. NHR-23 dependent collagen and hedgehog-related genes required for molting. Biochem Biophys Res Commun, 2011. |
| PubMed ID | 21910973 |
| Short Description | NHR-23 dependent collagen and hedgehog-related genes required for molting. GEO Record: GSE32031 Platform: GPL200 Download gene-centric, log2 transformed data: WBPaper00040185.ce.mr.csv |
| # of Conditions | 6 |
Full Description
|
NHR-23, a conserved member of the nuclear receptor family of transcription factors, is required for normal development in Caenorhabditis elegans where it plays a critical role in growth and molting. In a search for NHR-23 dependent genes, we performed whole genome comparative expression microarrays on both control and nhr-23 inhibited synchronized larvae. Genes that decreased in response to nhr-23 RNAi included several collagen genes. Unexpectedly, several hedgehog-related genes were also down-regulated after nhr-23 RNAi. A homozygous nhr-23 deletion allele was used to confirm the RNAi knockdown phenotypes and the changes in gene expression. Our results indicate that NHR-23 is a critical co-regulator of functionally linked genes involved in growth and molting and reveal evolutionary parallels among the ecdysozoa. Experimental Details: WBPaper00040185:Control_rep1 WBPaper00040185:Control_rep2 WBPaper00040185:Control_rep3 WBPaper00040185:nhr-23(RNAi)_rep1 WBPaper00040185:nhr-23(RNAi)_rep2 WBPaper00040185:nhr-23(RNAi)_rep3. |
|
Tags
|
Contact: help (at) wormbase.org
