| Citation | Chen C, Samuel TK, Krause M, Dailey HA, Hamza I. Heme utilization in the Caenorhabditis elegans hypodermal cells is facilitated by heme-responsive gene-2. J Biol Chem, 2012. |
| PubMed ID | 22303006 |
| Short Description | Heme utilization in the Caenorhabditis elegans hypodermal cells is facilitated by heme-responsive gene-2. GEO Record: GSE34471 Platform: GPL200 Download gene-centric, log2 transformed data: WBPaper00040730.ce.mr.csv |
| # of Conditions | 12 |
Full Description
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The roundworm Caenorhabditis elegans is a heme auxotroph that requires the coordinated actions of HRG-1 heme permeases to transport environmental heme into the intestine and HRG-3, a secreted protein, to deliver intestinal heme to other tissues including the embryo. Here we show that heme homeostasis in the extraintestinal hypodermal tissue was facilitated by the transmembrane protein HRG-2. Systemic heme deficiency up-regulated hrg-2 mRNA expression over 200-fold in the main body hypodermal syncytium, hyp 7. HRG-2 is a type I membrane protein that binds heme and localizes to the endoplasmic reticulum and apical plasma membrane. Cytochrome heme profiles are aberrant in HRG-2-deficient worms, a phenotype that was partially suppressed by heme supplementation. A heme-deficient yeast strain, ectopically expressing worm HRG-2, revealed significantly improved growth at submicromolar concentrations of exogenous heme. Taken together, our results implicate HRG-2 as a facilitator of heme utilization in the Caenorhabditis elegans hypodermis and provide a mechanism for the regulation of heme homeostasis in an extraintestinal tissue. Experimental Details: WBPaper00040730:4uM-Heme-N2_rep1 WBPaper00040730:4uM-Heme-N2_rep2 WBPaper00040730:4uM-Heme-N2_rep3 WBPaper00040730:20uM-Heme-N2_rep1 WBPaper00040730:20uM-Heme-N2_rep2 WBPaper00040730:20uM-Heme-N2_rep3 WBPaper00040730:4uM-Heme-hrg-2(tm3978)_rep1 WBPaper00040730:4uM-Heme-hrg-2(tm3978)_rep2 WBPaper00040730:4uM-Heme-hrg-2(tm3978)_rep3 WBPaper00040730:20uM-Heme-hrg-2(tm3978)_rep1 WBPaper00040730:20uM-Heme-hrg-2(tm3978)_rep2 WBPaper00040730:20uM-Heme-hrg-2(tm3978)_rep3. |
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