| Citation | Pietsch K, Saul N, Swain SC, Menzel R, Steinberg CE, Sturzenbaum SR. Meta-Analysis of Global Transcriptomics Suggests that Conserved Genetic Pathways are Responsible for Quercetin and Tannic Acid Mediated Longevity in C. elegans. Front Genet, 2012. |
| PubMed ID | 22493606 |
| Short Description | Meta-Analysis of Global Transcriptomics Suggests that Conserved Genetic Pathways are Responsible for Quercetin and Tannic Acid Mediated Longevity in C. elegans. GEO Record: GSE35354 Platform: GPL200 Download gene-centric, log2 transformed data: WBPaper00040963.ce.mr.csv |
| # of Conditions | 24 |
Full Description
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Recent research has highlighted that the polyphenols Quercetin and Tannic acid are capable of extending the lifespan of Caenorhabditis elegans. To gain a deep understanding of the underlying molecular genetics, we analyzed the global transcriptional patterns of nematodes exposed to three concentrations of Quercetin or Tannic acid, respectively. By means of an intricate meta-analysis it was possible to compare the transcriptomes of polyphenol exposure to recently published datasets derived from (i) longevity mutants or (ii) infection. This detailed comparative in silico analysis facilitated the identification of compound specific and overlapping transcriptional profiles and allowed the prediction of putative mechanistic models of Quercetin and Tannic acid mediated longevity. Lifespan extension due to Quercetin was predominantly driven by the metabolome, TGF-beta signaling, Insulin-like signaling, and the p38 MAPK pathway and Tannic acid's impact involved, in part, the amino acid metabolism and was modulated by the TGF-beta and the p38 MAPK pathways. DAF-12, which integrates TGF-beta and Insulin-like downstream signaling, and genetic players of the p38 MAPK pathway therefore seem to be crucial regulators for both polyphenols. Taken together, this study underlines how meta-analyses can provide an insight of molecular events that go beyond the traditional categorization into gene ontology-terms and Kyoto encyclopedia of genes and genomes-pathways. It also supports the call to expand the generation of comparative and integrative databases, an effort that is currently still in its infancy. Experimental Details: WBPaper00040963:Q_Control_rep1 WBPaper00040963:Q_Control_rep2 WBPaper00040963:Q_Control_rep3 WBPaper00040963:Q_50_rep1 WBPaper00040963:Q_50_rep2 WBPaper00040963:Q_50_rep3 WBPaper00040963:Q_100_rep1 WBPaper00040963:Q_100_rep2 WBPaper00040963:Q_100_rep3 WBPaper00040963:Q_200_rep1 WBPaper00040963:Q_200_rep2 WBPaper00040963:Q_200_rep3 WBPaper00040963:TA_Control_rep1 WBPaper00040963:TA_Control_rep2 WBPaper00040963:TA_Control_rep3 WBPaper00040963:TA_100_rep1 WBPaper00040963:TA_100_rep2 WBPaper00040963:TA_100_rep3 WBPaper00040963:TA_200_rep1 WBPaper00040963:TA_200_rep2 WBPaper00040963:TA_200_rep3 WBPaper00040963:TA_300_rep1 WBPaper00040963:TA_300_rep2 WBPaper00040963:TA_300_rep3. |
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