| Citation | Sahu SN, Lewis J, Patel I, Bozdag S, Lee JH, Leclerc JE, Cinar HN. Genomic analysis of immune response against Vibrio cholerae hemolysin in Caenorhabditis elegans. PLoS One, 2012. |
| PubMed ID | 22675448 |
| Short Description | Genomic analysis of immune response against Vibrio cholerae hemolysin in Caenorhabditis elegans. GEO Record: GSE34026 Platform: GPL200 Download gene-centric, log2 transformed data: WBPaper00041163.ce.mr.csv |
| # of Conditions | 15 |
Full Description
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Vibrio cholerae cytolysin (VCC) is among the accessory V. cholerae virulence factors that may contribute to disease pathogenesis in humans. VCC, encoded by hlyA gene, belongs to the most common class of bacterial toxins, known as pore-forming toxins (PFTs). V. cholerae infects and kills Caenorhabditis elegans via cholerae toxin independent manner. VCC is required for the lethality, growth retardation and intestinal cell vacuolation during the infection. However, little is known about the host gene expression responses against VCC. To address this question we performed a microarray study in C. elegans exposed to V. cholerae strains with intact and deleted hlyA genes.Many of the VCC regulated genes identified, including C-type lectins, Prion-like (glutamine [Q]/asparagine [N]-rich)-domain containing genes, genes regulated by insulin/IGF-1-mediated signaling (IIS) pathway, were previously reported as mediators of innate immune response against other bacteria in C. elegans. Protective function of the subset of the genes up-regulated by VCC was confirmed using RNAi. By means of a machine learning algorithm called FastMEDUSA, we identified several putative VCC induced immune regulatory transcriptional factors and transcription factor binding motifs. Our results suggest that VCC is a major virulence factor, which induces a wide variety of immune response- related genes during V. cholerae infection in C. elegans. Experimental Details: WBPaper00041163:N2_CVD109_rep1 WBPaper00041163:N2_CVD109_rep2 WBPaper00041163:N2_CVD109_rep3 WBPaper00041163:N2_CVD110_rep1 WBPaper00041163:N2_CVD110_rep2 WBPaper00041163:N2_CVD110_rep3 WBPaper00041163:N2_hemolysin-E7946_rep1 WBPaper00041163:N2_hemolysin-E7946_rep2 WBPaper00041163:N2_hemolysin-E7946_rep3 WBPaper00041163:N2_E7946_rep1 WBPaper00041163:N2_E7946_rep2 WBPaper00041163:N2_E7946_rep3 WBPaper00041163:N2_OP50_rep1 WBPaper00041163:N2_OP50_rep2 WBPaper00041163:N2_OP50_rep3. |
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