SPELL - Nematode

Citation	Quach TK, Chou HT, Wang K, Milledge GZ, Johnson CM. Genome-wide microarrray analysis reveals roles for the REF-1 family member HLH-29 in ferritin synthesis and peroxide stress response. PLoS One, 2013.
PubMed ID	23533643
Short Description	Genome-wide microarrray analysis reveals roles for the REF-1 family member HLH-29 in ferritin synthesis and peroxide stress response. GEO Record: GSE65417 Platform: GPL200 Download gene-centric, log2 transformed data: WBPaper00042178.ce.mr.csv
# of Conditions	9
Full Description	In Caenorhabditis elegans, the six proteins that make up the REF-1 family have been identified as functional homologs of the Hairy/Enhancer of Split (HES) proteins. These transcription factors act in both Notch dependent and Notch-independent pathways to regulate embryonic events during development; however, their post-embryonic functions are not well defined. As a first step toward understanding how the REF-1 family works together to coordinate post-embryonic events, we used gene expression microarray analysis to identify transcriptional targets of HLH-29 in L4/young adult stage animals. Here we show that HLH-29 targets are genes needed for the regulation of growth and lifespan, including genes required for oxidative stress response and fatty acid metabolism, and the ferritin genes, ftn-1 and ftn-2. We show that HLH-29 regulates ftn-1 expression via promoter sequences upstream of the iron-dependent element that is recognized by the hypoxia inducible factor, HIF-1. Additionally, hlh-29 mutants are more resistant to peroxide stress than wild-type animals and ftn-1(RNAi) animals, even in the presence of excess iron. Finally we show that HLH-29 acts parallel to DAF-16 but upstream of the microphthalmia transcription factor ortholog, HLH-30, to regulate ftn-1 expression under normal growth conditions. Experimental Details: WBPaper00042178:N2_rep1 WBPaper00042178:N2_rep2 WBPaper00042178:N2_rep3 WBPaper00042178:hlh-25(ok1710)_rep1 WBPaper00042178:hlh-25(ok1710)_rep2 WBPaper00042178:hlh-25(ok1710)_rep3 WBPaper00042178:hlh-29(tm284)_rep1 WBPaper00042178:hlh-29(tm284)_rep2 WBPaper00042178:hlh-29(tm284)_rep3.
Tags	Method: microarray, Species: Caenorhabditis elegans, Topic: WT vs. mutant