SPELL - Nematode

Citation	MacNeil LT, Watson E, Arda HE, Zhu LJ, Walhout AJ. Diet-induced developmental acceleration independent of TOR and insulin in C. elegans. Cell, 2013.
PubMed ID	23540701
Short Description	Diet-induced developmental acceleration independent of TOR and insulin in C. elegans. GEO Record: GSE43959 GSE43953 GSE43954 Platform: GPL200 Download gene-centric, log2 transformed data: WBPaper00042258.ce.mr.csv
# of Conditions	18
Full Description	Dietary composition has major effects on physiology. Here, we show that developmental rate, reproduction, and lifespan are altered in C.elegans fed Comamonas DA1877 relative to those fed a standard E.coli OP50 diet. We identify a set of genes that change in expression in response to this diet and use the promoter of one of these (acdh-1) as a dietary sensor. Remarkably, the effects on transcription anddevelopment occur even when Comamonas DA1877 is diluted with another diet, suggesting that Comamonas DA1877 generates a signal that is sensed by the nematode. Surprisingly, the developmental effect is independent from TOR and insulin signaling. Rather, Comamonas DA1877 affects cyclic gene expression during molting, likely through the nuclear hormone receptor NHR-23. Altogether, our findings indicate that different bacteria elicit various responses via distinct mechanisms, which has implications for diseases such as obesity and the interactions between the human microbiome and intestinal cells. Experimental Details: WBPaper00042258:N2_gravid-adult_DA1877_rep1 WBPaper00042258:N2_gravid-adult_DA1877_rep2 WBPaper00042258:N2_gravid-adult_DA1877_rep3 WBPaper00042258:N2_gravid-adult_diluted-DA1877_rep1 WBPaper00042258:N2_gravid-adult_diluted-DA1877_rep2 WBPaper00042258:N2_gravid-adult_diluted-DA1877_rep3 WBPaper00042258:N2_gravid-adult_OP50_rep1 WBPaper00042258:N2_gravid-adult_OP50_rep2 WBPaper00042258:N2_gravid-adult_OP50_rep3 WBPaper00042258:N2_young-adult_OP50_rep1 WBPaper00042258:N2_young-adult_OP50_rep2 WBPaper00042258:N2_young-adult_OP50_rep3 WBPaper00042258:N2_young-adult_HT115_rep1 WBPaper00042258:N2_young-adult_HT115_rep2 WBPaper00042258:N2_young-adult_HT115_rep3 WBPaper00042258:N2_young-adult_DA1877_rep1 WBPaper00042258:N2_young-adult_DA1877_rep2 WBPaper00042258:N2_young-adult_DA1877_rep3.
Tags	Method: microarray, Species: Caenorhabditis elegans, Topic: unclassified