SPELL - Nematode

Citation	Bond MR, Ghosh SK, Wang P, Hanover JA. Conserved nutrient sensor O-GlcNAc transferase is integral to C. elegans pathogen-specific immunity. PLoS One, 2014.
PubMed ID	25474640
Short Description	Conserved nutrient sensor O-GlcNAc transferase is integral to C. elegans pathogen-specific immunity. GEO Record: GSE53732 Platform: GPL200 Download gene-centric, log2 transformed data: WBPaper00046083.ce.mr.csv
# of Conditions	36
Full Description	Discriminating pathogenic bacteria from bacteria used as a food source is key to Caenorhabidits elegans immunity. Using mutants defective in the enzymes of O-linked N-acetylglucosamine (O-GlcNAc) cycling, we examined the role of this nutrient-sensing pathway in the C. elegans innate immune response. Genetic analysis showed that deletion of O-GlcNAc transferase (ogt-1) yielded animals hypersensitive to the human pathogen S. aureus but not to P. aeruginosa. Genetic interaction studies revealed that nutrient-responsive OGT-1 acts through the conserved -catenin (BAR-1) pathway and in concert with p38 MAPK (PMK-1) to modulate the immune response to S. aureus. Moreover, whole genome transcriptional profiling revealed that O-GlcNAc cycling mutants exhibited deregulation of unique stress- and immune-responsive genes. The participation of nutrient sensor OGT-1 in an immunity module evolutionarily conserved from C. elegans to humans reveals an unexplored nexus between nutrient availability and a pathogen-specific immune response. Experimental Details: WBPaper00046083:pmk-1(km25)_OP50_sample1 WBPaper00046083:pmk-1(km25)_OP50_sample2 WBPaper00046083:pmk-1(km25)_OP50_sample3 WBPaper00046083:ogt-1(ok1474)_OP50_sample4 WBPaper00046083:ogt-1(ok1474)_OP50_sample5 WBPaper00046083:ogt-1(ok1474)_OP50_sample6 WBPaper00046083:oga-1(ok1207)_OP50_sample7 WBPaper00046083:oga-1(ok1207)_OP50_sample8 WBPaper00046083:oga-1(ok1207)_OP50_sample9 WBPaper00046083:N2_OP50_sample10 WBPaper00046083:N2_OP50_sample11 WBPaper00046083:N2_OP50_sample12 WBPaper00046083:pmk-1(km25)_S.aureus_sample13 WBPaper00046083:pmk-1(km25)_S.aureus_sample14 WBPaper00046083:pmk-1(km25)_S.aureus_sample15 WBPaper00046083:ogt-1(ok1474)_S.aureus_sample16 WBPaper00046083:ogt-1(ok1474)_S.aureus_sample17 WBPaper00046083:ogt-1(ok1474)_S.aureus_sample18 WBPaper00046083:oga-1(ok1207)_S.aureus_sample19 WBPaper00046083:oga-1(ok1207)_S.aureus_sample20 WBPaper00046083:oga-1(ok1207)_S.aureus_sample21 WBPaper00046083:N2_S.aureus_sample22 WBPaper00046083:N2_S.aureus_sample23 WBPaper00046083:N2_S.aureus_sample24 WBPaper00046083:pmk-1(km25)_P.aeruginosa_sample25 WBPaper00046083:pmk-1(km25)_P.aeruginosa_sample26 WBPaper00046083:pmk-1(km25)_P.aeruginosa_sample27 WBPaper00046083:ogt-1(ok1474)_P.aeruginosa_sample28 WBPaper00046083:ogt-1(ok1474)_P.aeruginosa_sample29 WBPaper00046083:ogt-1(ok1474)_P.aeruginosa_sample30 WBPaper00046083:oga-1(ok1207)_P.aeruginosa_sample31 WBPaper00046083:oga-1(ok1207)_P.aeruginosa_sample32 WBPaper00046083:oga-1(ok1207)_P.aeruginosa_sample33 WBPaper00046083:N2_P.aeruginosa_sample34 WBPaper00046083:N2_P.aeruginosa_sample35 WBPaper00046083:N2_P.aeruginosa_sample36.
Tags	Method: microarray, Species: Caenorhabditis elegans, Topic: innate immune response