SPELL - Nematode

Citation	Spiegler V, Hensel A, SeggewiB J, Lubisch M, Liebau E. Transcriptome analysis reveals molecular anthelmintic effects of procyanidins in C. elegans. PLoS One, 2017.
PubMed ID	28926584
Short Description	Transcriptome analysis reveals molecular anthelmintic effects of procyanidins in C. elegans. GEO Record: GSE101680 Platform: GPL19230 Download gene-centric, log2 transformed data: WBPaper00053092.ce.mr.csv
# of Conditions	15
Full Description	Worldwide, more than 1 billion people are affected by infestations with soil-transmitted helminths and also in veterinary medicine helminthiases are a severe threat to livestock due to emerging resistances against the common anthelmintics. Proanthocyanidins have been increasingly investigated for their anthelmintic properties, however, except for an interaction with certain proteins of the nematodes, not much is known about their mode of action. To investigate the anthelmintic activity on a molecular level, a transcriptome analysis was performed in Caenorhabditis elegans after treatment with purified and fully characterized oligomeric procyanidins (OPC). The OPCs had previously been obtained from a hydro-ethanolic (1:1) extract from the leaves of Combretum mucronatum, a plant which is traditionally used in West Africa for the treatment of helminthiasis, therefore, also the crude extract was included in the study. Significant changes in differential gene expression were observed mainly for proteins related to the intestine, many of which were located extracellularly or within cellular membranes. Among the up-regulated genes, several hitherto undescribed orthologues of structural proteins in humans were identified, but also genes that are potentially involved in the worms' defense against tannins. For example, T22D1.2, an orthologue of human basic salivary proline-rich protein (PRB) 2, and numr-1 (nuclear localized metal responsive) were found to be strongly up-regulated. Down-regulated genes were mainly associated with lysosomal activity, glycoside hydrolysis or the worms' innate immune response. No major differences were found between the groups treated with purified OPCs versus the crude extract. Investigations using GFP reporter gene constructs of T22D1.2 and numr-1 corroborated the intestine as the predominant site of the anthelmintic activity. The current findings support previous hypotheses of OPCs interacting with intestinal surface proteins and provide the first insights into the nematode's response to OPCs on a molecular level as a base for the identification of future drug targets. Experimental Details: WBPaper00053092:C.mucronatum-H3-fraction_0.02_rep1 WBPaper00053092:C.mucronatum-H3-fraction_2_rep1 WBPaper00053092:Control_rep1 WBPaper00053092:C.mucronatum-H3-fraction_0.02_rep2 WBPaper00053092:C.mucronatum-H3-fraction_0.2_rep1 WBPaper00053092:C.mucronatum-H3-fraction_2_rep2 WBPaper00053092:C.mucronatum-extract_0.2_rep1 WBPaper00053092:Control_rep2 WBPaper00053092:C.mucronatum-H3-fraction_0.02_rep3 WBPaper00053092:C.mucronatum-H3-fraction_0.2_rep2 WBPaper00053092:C.mucronatum-H3-fraction_2_rep3 WBPaper00053092:C.mucronatum-extract_0.2_rep2 WBPaper00053092:Control_rep3 WBPaper00053092:C.mucronatum-H3-fraction_0.2_rep3 WBPaper00053092:C.mucronatum-extract_0.2_rep3.
Tags	Method: microarray, Species: Caenorhabditis elegans, Topic: response to chemical