SPELL - Nematode

Citation	Hibshman JD, Doan AE, Moore BT, Kaplan RE, Hung A, Webster AK, Bhatt DP, Chitrakar R, Hirschey MD, Baugh LR. daf-16/FoxO promotes gluconeogenesis and trehalose synthesis during starvation to support survival. Elife, 2017.
PubMed ID	29063832
Short Description	daf-16/FoxO promotes gluconeogenesis and trehalose synthesis during starvation to support survival. GEO Record: GSE99201 Platform: GPL200 Download gene-centric, log2 transformed data: WBPaper00053236.ce.mr.csv
# of Conditions	12
Full Description	daf-16/FoxO is required to survive starvation in Caenorhabditis elegans, but how daf-16IFoxO promotes starvation resistance is unclear. We show that daf-16/FoxO restructures carbohydrate metabolism by driving carbon flux through the glyoxylate shunt and gluconeogenesis and into synthesis of trehalose, a disaccharide of glucose. Trehalose is a well-known stress protectant, capable of preserving membrane organization and protein structure during abiotic stress. Metabolomic, genetic, and pharmacological analyses confirm increased trehalose synthesis and further show that trehalose not only supports survival as a stress protectant but also serves as a glycolytic input. Furthermore, we provide evidence that metabolic cycling between trehalose and glucose is necessary for this dual function of trehalose. This work demonstrates that daf-16/FoxO promotes starvation resistance by shifting carbon metabolism to drive trehalose synthesis, which in turn supports survival by providing an energy source and acting as a stress protectant. Experimental Details: WBPaper00053236:N2_Fed_RepE WBPaper00053236:N2_Starved_RepE WBPaper00053236:daf-16(mgDf50)_Fed_RepE WBPaper00053236:daf-16(mgDf50)_Starved_RepE WBPaper00053236:N2_Fed_RepF WBPaper00053236:N2_Starved_RepF WBPaper00053236:daf-16(mgDf50)_Fed_RepF WBPaper00053236:daf-16(mgDf50)_Starved_RepF WBPaper00053236:N2_Fed_RepG WBPaper00053236:N2_Starved_RepG WBPaper00053236:daf-16(mgDf50)_Fed_RepG WBPaper00053236:daf-16(mgDf50)_Starved_RepG.
Tags	Method: microarray, Species: Caenorhabditis elegans, Topic: response to starvation