| Citation | Koh JH, Wang L, Beaudoin-Chabot C, Thibault G. Lipid bilayer stress-activated IRE-1 modulates autophagy during endoplasmic reticulum stress. J Cell Sci, 2018. |
| PubMed ID | 30333136 |
| Short Description | Lipid bilayer stress-activated IRE-1 modulates autophagy during endoplasmic reticulum stress. GEO Record: GSE99763 Platform: GPL19230 Download gene-centric, log2 transformed data: WBPaper00055482.ce.mr.csv |
| # of Conditions | 30 |
Full Description
|
Metabolic disorders such as nonalcoholic fatty liver disease (NAFLD) are emerging epidemics that affect the global population. One facet of these disorders is attributed to the disturbance of membrane lipid composition. Perturbation of endoplasmic reticulum (ER) homeostasis through alteration in membrane phospholipids activates the unfolded protein response (UPR) and causes dramatic transcriptional and translational changes in the cell. To restore cellular homeostasis, the three highly conserved UPR transducers ATF6, IRE1, and PERK mediate adaptive responses upon ER stress. The homeostatic UPR cascade is well characterised under conditions of proteotoxic stress, but much less so under lipid bilayer stress induced-UPR. Disrupted phosphatidylcholine (PC) synthesis in C. elegans causes lipid bilayer stress, lipid droplet accumulation and ER stress induction. Transcriptional profiling of PC-deficient worms shows a unique subset of genes regulated in a UPR-dependent manner that is independent from proteotoxic stress. Among these, we show that autophagy is modulated through the conserved IRE-1/XBP-1 axis, strongly suggesting of the importance of autophagy in maintaining cellular homeostasis during lipid bilayer induced-UPR. Experimental Details: WBPaper00055482:N2_VectorControl_rep1 WBPaper00055482:N2_VectorControl_rep2 WBPaper00055482:N2_VectorControl_rep3 WBPaper00055482:N2_pmt-2(RNAi)_rep1 WBPaper00055482:N2_pmt-2(RNAi)_rep2 WBPaper00055482:N2_pmt-2(RNAi)_rep3 WBPaper00055482:N2_Tunicamycin_rep1 WBPaper00055482:N2_Tunicamycin_rep2 WBPaper00055482:N2_Tunicamycin_rep3 WBPaper00055482:atf-6(ok551)_VectorControl_rep1 WBPaper00055482:atf-6(ok551)_VectorControl_rep2 WBPaper00055482:atf-6(ok551)_VectorControl_rep3 WBPaper00055482:atf-6(ok551)_pmt-2(RNAi)_rep1 WBPaper00055482:atf-6(ok551)_pmt-2(RNAi)_rep2 WBPaper00055482:atf-6(ok551)_pmt-2(RNAi)_rep3 WBPaper00055482:ire-1(ok799)_VectorControl_rep1 WBPaper00055482:ire-1(ok799)_VectorControl_rep2 WBPaper00055482:ire-1(ok799)_VectorControl_rep3 WBPaper00055482:ire-1(ok799)_pmt-2(RNAi)_rep1 WBPaper00055482:ire-1(ok799)_pmt-2(RNAi)_rep2 WBPaper00055482:ire-1(ok799)_pmt-2(RNAi)_rep3 WBPaper00055482:pek-1(ok275)_VectorControl_rep1 WBPaper00055482:pek-1(ok275)_VectorControl_rep2 WBPaper00055482:pek-1(ok275)_VectorControl_rep3 WBPaper00055482:pek-1(ok275)_pmt-2(RNAi)_rep1 WBPaper00055482:pek-1(ok275)_pmt-2(RNAi)_rep2 WBPaper00055482:pek-1(ok275)_pmt-2(RNAi)_rep3 WBPaper00055482:pmt-2(vc1952)_VectorControl_rep1 WBPaper00055482:pmt-2(vc1952)_VectorControl_rep2 WBPaper00055482:pmt-2(vc1952)_VectorControl_rep3. |
|
Tags
|
Contact: help (at) wormbase.org
