SPELL - Nematode

Citation	Ballesteros C, Tritten L, O'Neill M, Burkman E, Zaky WI, Xia J, Moorhead A, Williams SA, Geary TG. The Effect of In Vitro Cultivation on the Transcriptome of Adult Brugia malayi. PLoS Negl Trop Dis, 2016.
PubMed ID	26727204
Short Description	The Effect of In Vitro Cultivation on the Transcriptome of Adult Brugia malayi. GEO Record: N.A. Platform: N.A. Download gene-centric, log2 transformed data: WBPaper00049086.bma.rs.csv
# of Conditions	24
Full Description	BACKGROUND: Filarial nematodes cause serious and debilitating infections in human populations of tropical countries, contributing to an entrenched cycle of poverty. Only one human filarial parasite, Brugia malayi, can be maintained in rodents in the laboratory setting. It has been a widely used model organism in experiments that employ culture systems, the impact of which on the worms is unknown. METHODOLOGY/PRINCIPAL FINDINGS: Using Illumina RNA sequencing, we characterized changes in gene expression upon in vitro maintenance of adult B. malayi female worms at four time points: immediately upon removal from the host, immediately after receipt following shipment, and after 48 h and 5 days in liquid culture media. The dramatic environmental change and the 24 h time lapse between removal from the host and establishment in culture caused a globally dysregulated gene expression profile. We found a maximum of 562 differentially expressed genes based on pairwise comparison between time points. After an initial shock upon removal from the host and shipping, a few stress fingerprints remained after 48 h in culture and until the experiment was stopped. This was best illustrated by a strong and persistent up-regulation of several genes encoding cuticle collagens, as well as serpins. CONCLUSIONS/SIGNIFICANCE: These findings suggest that B. malayi can be maintained in culture as a valid system for pharmacological and biological studies, at least for several days after removal from the host and adaptation to the new environment. However, genes encoding several stress indicators remained dysregulated until the experiment was stopped. Experimental Details: RNASeq.brugia.WBStrain00041073.WBls:0000083.Female.WBbt:0007833.SRP063061.SRX1176947 RNASeq.brugia.WBStrain00041073.WBls:0000083.Female.WBbt:0007833.SRP063061.SRX1176948 RNASeq.brugia.WBStrain00041073.WBls:0000083.Female.WBbt:0007833.SRP063061.SRX1176949 RNASeq.brugia.WBStrain00041073.WBls:0000083.Female.WBbt:0007833.SRP063061.SRX1176950 RNASeq.brugia.WBStrain00041073.WBls:0000083.Female.WBbt:0007833.SRP063061.SRX1176951 RNASeq.brugia.WBStrain00041073.WBls:0000083.Female.WBbt:0007833.SRP063061.SRX1176952 RNASeq.brugia.WBStrain00041073.WBls:0000083.Female.WBbt:0007833.SRP063061.SRX1176953 RNASeq.brugia.WBStrain00041073.WBls:0000083.Female.WBbt:0007833.SRP063061.SRX1176954 RNASeq.brugia.WBStrain00041073.WBls:0000083.Female.WBbt:0007833.SRP063061.SRX1176955 RNASeq.brugia.WBStrain00041073.WBls:0000083.Female.WBbt:0007833.SRP063061.SRX1176956 RNASeq.brugia.WBStrain00041073.WBls:0000083.Female.WBbt:0007833.SRP063061.SRX1176957 RNASeq.brugia.WBStrain00041073.WBls:0000083.Female.WBbt:0007833.SRP063061.SRX1176958 RNASeq.brugia.WBStrain00041073.WBls:0000083.Female.WBbt:0007833.SRP063061.SRX1176959 RNASeq.brugia.WBStrain00041073.WBls:0000083.Female.WBbt:0007833.SRP063061.SRX1176960 RNASeq.brugia.WBStrain00041073.WBls:0000083.Female.WBbt:0007833.SRP063061.SRX1176961 RNASeq.brugia.WBStrain00041073.WBls:0000083.Female.WBbt:0007833.SRP063061.SRX1176962 RNASeq.brugia.WBStrain00041073.WBls:0000083.Female.WBbt:0007833.SRP063061.SRX1176963 RNASeq.brugia.WBStrain00041073.WBls:0000083.Female.WBbt:0007833.SRP063061.SRX1176964 RNASeq.brugia.WBStrain00041073.WBls:0000083.Female.WBbt:0007833.SRP063061.SRX1176965 RNASeq.brugia.WBStrain00041073.WBls:0000083.Female.WBbt:0007833.SRP063061.SRX1176966 RNASeq.brugia.WBStrain00041073.WBls:0000083.Female.WBbt:0007833.SRP063061.SRX1176967 RNASeq.brugia.WBStrain00041073.WBls:0000083.Female.WBbt:0007833.SRP063061.SRX1176968 RNASeq.brugia.WBStrain00041073.WBls:0000083.Female.WBbt:0007833.SRP063061.SRX1176969 RNASeq.brugia.WBStrain00041073.WBls:0000083.Female.WBbt:0007833.SRP063061.SRX1176970.
Tags	Method: RNAseq, Species: Brugia malayi, Topic: developmental time course