| Citation | Revtovich AV, Lee R, Kirienko NV. Interplay between mitochondria and diet mediates pathogen and stress resistance in Caenorhabditis elegans. PLoS Genet, 2019. |
| PubMed ID | 30865620 |
| Short Description | Interplay between mitochondria and diet mediates pathogen and stress resistance in Caenorhabditis elegans. GEO Record: GSE97678 Platform: GPL200 Download gene-centric, log2 transformed data: WBPaper00056391.ce.mr.csv |
| # of Conditions | 6 |
Full Description
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Diet is a crucial determinant of organismal biology; interactions between the host, its diet, and its microbiota are critical to determining the health of an organism. A variety of genetic and biochemical means were used to assay stress sensitivity in C. elegans reared on two standard laboratory diets: E. coli OP50, the most commonly used food for C. elegans, or E. coli HT115, which is typically used for RNAi-mediated gene knockdown. We demonstrated that the relatively subtle shift to a diet of E. coli HT115 had a dramatic impact on C. elegans's survival after exposure to pathogenic or abiotic stresses. Interestingly, this was independent of canonical host defense pathways. Instead the change arises from improvements in mitochondrial health, likely due to alleviation of a vitamin B12 deficiency exhibited by worms reared on an E. coli OP50 diet. Increasing B12 availability, by feeding on E. coli HT115, supplementing E. coli OP50 with exogenous vitamin B12, or overexpression of the B12 transporter, improved mitochondrial homeostasis and increased resistance. Loss of the methylmalonyl-CoA mutase gene mmcm-1/MUT, which requires vitamin B12 as a cofactor, abolished these improvements, establishing a genetic basis for the E. coli OP50-incurred sensitivity. Our study forges a mechanistic link between a dietary deficiency (nutrition/microbiota) and a physiological consequence (host sensitivity), using the host-microbiota-diet framework. Experimental Details: WBPaper00056391:glp-4_OP50_repA WBPaper00056391:glp-4_OP50_repB WBPaper00056391:glp-4_OP50_repC WBPaper00056391:glp-4_HT115_repA WBPaper00056391:glp-4_HT115_repB WBPaper00056391:glp-4_HT115_repC. |
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