SPELL - Nematode

Citation	Finger F, Ottens F, Springhorn A, Drexel T, Proksch L, Metz S, Cochella L, Hoppe T. Olfaction regulates organismal proteostasis and longevity via microRNA-dependent signaling. Nat Metab, 2019.
PubMed ID	31535080
Short Description	Olfaction regulates organismal proteostasis and longevity via microRNA-dependent signaling. GEO Record: GSE124300 Platform: GPL19230 Download gene-centric, log2 transformed data: WBPaper00058595.ce.mr.csv
# of Conditions	8
Full Description	The maintenance of proteostasis is crucial for any organism to survive and reproduce in an ever-changing environment, but its efficiency declines with age¹. Posttranscriptional regulators such as microRNAs control protein translation of target mRNAs with major consequences for development, physiology, and longevity^2,3. Here we show that food odor stimulates organismal proteostasis and promotes longevity in Caenorhabditis elegans through mir-71-mediated inhibition of tir-1 mRNA stability in olfactory AWC neurons. Screening a collection of microRNAs that control aging³ we find that miRNA mir-71 regulates lifespan and promotes ubiquitin-dependent protein turnover, particularly in the intestine. We show that mir-71 directly inhibits the toll receptor domain protein TIR-1 in AWC olfactory neurons and that disruption of mir-71/tir-1 or loss of AWC olfactory neurons eliminates the influence of food source on proteostasis. mir-71-mediated regulation of TIR-1 controls chemotactic behavior and is regulated by odor. Thus, odor perception influences cell-type specific miRNA-target interaction to regulate organismal proteostasis and longevity. We anticipate that the proposed mechanism of food perception will stimulate further research on neuroendocrine brain-to-gut communication and may open the possibility for therapeutic interventions to improve proteostasis and organismal health via the sense of smell, with potential implication for obesity, diabetes and aging. Experimental Details: WBPaper00058595:N2_rep1 WBPaper00058595:N2_rep2 WBPaper00058595:N2_rep3 WBPaper00058595:N2_rep4 WBPaper00058595:mir-71(n4115)_rep1 WBPaper00058595:mir-71(n4115)_rep2 WBPaper00058595:mir-71(n4115)_rep3 WBPaper00058595:mir-71(n4115)_rep4.
Tags	Method: microarray, Species: Caenorhabditis elegans, Topic: WT vs. mutant