SPELL - Nematode

Citation	Segref A, Vakkayil KL, Padvitski T, Li Q, Kroef V, Lormann J, Korner L, Finger F, Hoppe T. Thermosensation in Caenorhabditis elegans is linked to ubiquitin-dependent protein turnover via insulin and calcineurin signalling. Nat Commun, 2022.
PubMed ID	36198694
Short Description	Thermosensation in Caenorhabditis elegans is linked to ubiquitin-dependent protein turnover via insulin and calcineurin signalling. GEO Record: GSE142371 Platform: GPL19230 Download gene-centric, log2 transformed data: WBPaper00064565.ce.mr.csv
# of Conditions	8
Full Description	Organismal physiology and survival are influenced by environmental conditions and linked to protein quality control. Proteome integrity is achieved by maintaining an intricate balance between protein folding and degradation. In Caenorhabditis elegans, acute heat stress determines cell non-autonomous regulation of chaperone levels. However, how the perception of environmental changes, including physiological temperature, affects protein degradation remains largely unexplored. Here, we show that loss-of-function of dyf-1 in Caenorhabditis elegans associated with dysfunctional sensory neurons leads to defects in both temperature perception and thermal adaptation of the ubiquitin/proteasome system centered on thermosensory AFD neurons. Impaired perception of moderate temperature changes worsens ubiquitin-dependent proteolysis in intestinal cells. Brain-gut communication regulating protein turnover is mediated by upregulation of the insulin-like peptide INS-5 and inhibition of the calcineurin-regulated forkhead-box transcription factor DAF-16/FOXO. Our data indicate that perception of ambient temperature and its neuronal integration is important for the control of proteome integrity in complex organisms. Experimental Details: WBPaper00064565:dyf-1(mn335)_rep1 WBPaper00064565:dyf-1(mn335)_rep2 WBPaper00064565:dyf-1(mn335)_rep3 WBPaper00064565:dyf-1(mn335)_rep4 WBPaper00064565:N2_rep1 WBPaper00064565:N2_rep2 WBPaper00064565:N2_rep3 WBPaper00064565:N2_rep4.
Tags	Method: microarray, Species: Caenorhabditis elegans, Topic: WT vs. mutant