SPELL - Nematode

Citation	Liu YJ, Janssens GE, McIntyre RL, Molenaars M, Kamble R, Gao AW, Jongejan A, Weeghel MV, MacInnes AW, Houtkooper RH. Glycine promotes longevity in Caenorhabditis elegans in a methionine cycle-dependent fashion. PLoS Genet, 2019.
PubMed ID	30845140
Short Description	Glycine promotes longevity in Caenorhabditis elegans in a methionine cycle-dependent fashion. GEO Record: GSE117581 Platform: GPL21109 Download gene-centric, log2 transformed data: WBPaper00056330.ce.mr.csv
# of Conditions	8
Full Description	The deregulation of metabolism is a hallmark of aging. As such, changes in the expression of metabolic genes and the profiles of amino acid levels are features associated with aging animals. We previously reported that the levels of most amino acids decline with age in Caenorhabditis elegans (C. elegans). Glycine, in contrast, substantially accumulates in aging C. elegans. In this study we show that this is coupled to a decrease in gene expression of enzymes important for glycine catabolism. We further show that supplementation of glycine significantly prolongs C. elegans lifespan, and early adulthood is important for its salutary effects. Moreover, supplementation of glycine ameliorates specific transcriptional changes that are associated with aging. Glycine feeds into the methionine cycle. We find that mutations in components of this cycle, methionine synthase (metr-1) and S-adenosylmethionine synthetase (sams-1), completely abrogate glycine-induced lifespan extension. Strikingly, the beneficial effects of glycine supplementation are conserved when we supplement with serine, which also feeds into the methionine cycle. RNA-sequencing reveals a similar transcriptional landscape in serine- and glycine-supplemented worms both demarked by widespread gene repression. Taken together, these data uncover a novel role of glycine in the deceleration of aging through its function in the methionine cycle. Experimental Details: WBPaper00056330:control_rep1 WBPaper00056330:control_rep2 WBPaper00056330:control_rep3 WBPaper00056330:control_rep4 WBPaper00056330:mrps-5(RNAi)_rep1 WBPaper00056330:mrps-5(RNAi)_rep2 WBPaper00056330:mrps-5(RNAi)_rep3 WBPaper00056330:mrps-5(RNAi)_rep4.
Tags	Method: microarray, Species: Caenorhabditis elegans, Topic: WT vs. mutant