SPELL - Nematode

Citation	Bai X, Huang LJ, Chen SW, Nebenfuhr B, Wysolmerski B, Wu JC, Olson SK, Golden A, Wang CW. Loss of the seipin gene perturbs eggshell formation in C. elegans. Development, 2020.
PubMed ID	32820022
Short Description	Loss of the seipin gene perturbs eggshell formation in C. elegans. GEO Record: GSE157800 Platform: GPL11346 Download gene-centric, log2 transformed data: WBPaper00060122.ce.mr.csv
# of Conditions	8
Full Description	SEIPIN, an evolutionary conserved protein, plays pivotal roles during lipid droplet (LD) biogenesis and is associated with various human diseases with unclear mechanisms. Here, we analyzed C. elegans mutants deleted of the sole SEIPIN gene, seip-1 Homozygous seip-1 mutants displayed penetrant embryonic lethality, which is caused by the disruption of the lipid-rich permeability barrier, the innermost layer of the C. elegans embryonic eggshell. In C. elegans oocytes and embryos, SEIP-1 is associated with LDs and crucial for controlling LD size and lipid homeostasis. The seip-1 deletion mutants reduced the ratio of polyunsaturated fatty acids (PUFAs) in their embryonic fatty acid pool. Interestingly, dietary supplementation of selected n-6 PUFAs rescued the embryonic lethality and defective permeability barrier. Accordingly, we propose that SEIP-1 may maternally regulate LD biogenesis and lipid homeostasis to orchestrate the formation of the permeability barrier for eggshell synthesis during embryogenesis. A lipodystrophy allele of seip-1 resulted in embryonic lethality as well and could be rescued by PUFA supplementation; these experiments support a great potential of using C. elegans to model SEIPIN-associated human diseases. Experimental Details: WBPaper00060122:N2_embryo_rep1 WBPaper00060122:N2_embryo_rep2 WBPaper00060122:seip-1(av109)_embryo_rep1 WBPaper00060122:seip-1(av109)_embryo_rep2 WBPaper00060122:N2_adult_worm_rep1 WBPaper00060122:N2_adult_worm_rep2 WBPaper00060122:seip-1(av109)_adult_worm_rep1 WBPaper00060122:seip-1(av109)_adult_worm_rep2.
Tags	Method: microarray, Species: Caenorhabditis elegans, Topic: WT vs. mutant