SPELL - Nematode

Citation	Chen D, Li PW, Goldstein BA, Cai W, Thomas EL, Chen F, Hubbard AE, Melov S, Kapahi P. Germline signaling mediates the synergistically prolonged longevity produced by double mutations in daf-2 and rsks-1 in C. elegans. Cell Rep, 2013.
PubMed ID	24332851
Short Description	Germline signaling mediates the synergistically prolonged longevity produced by double mutations in daf-2 and rsks-1 in C. elegans. GEO Record: GSE52340 Platform: GPL17925 Download gene-centric, log2 transformed data: WBPaper00044638.ce.mr.csv
# of Conditions	47
Full Description	Inhibition of DAF-2 (insulin-like growth factor 1 [IGF-1] receptor) or RSKS-1 (S6K), key molecules in the insulin/IGF-1 signaling (IIS) and target of rapamycin (TOR) pathways, respectively, extend lifespan in Caenorhabditis elegans. However, it has not been clear how and in which tissues they interact with each other to modulate longevity. Here, we demonstrate that a combination of mutations in daf-2 and rsks-1 produces a nearly 5-fold increase in longevity that is much greater than the sum of single mutations. This synergistic lifespan extension requires positive feedback regulation of DAF-16 (FOXO) via the AMP-activated protein kinase (AMPK) complex. Furthermore, we identify germline as the key tissue for this synergistic longevity. Moreover, germline-specific inhibition of rsks-1 activates DAF-16 in the intestine. Together, our findings highlight the importance of the germline in the significantly increased longevity produced by daf-2 rsks-1, which has important implications for interactions between the two major conserved longevity pathways in more complex organisms. Experimental Details: WBPaper00044638:N2_rep1 WBPaper00044638:N2_rep2 WBPaper00044638:N2_rep8 WBPaper00044638:N2_rep3 WBPaper00044638:N2_rep4 WBPaper00044638:N2_rep5 WBPaper00044638:N2_rep6 WBPaper00044638:N2_rep7 WBPaper00044638:N2_rep10 WBPaper00044638:rsks-1(ok1255)_rep1 WBPaper00044638:rsks-1(ok1255)_rep2 WBPaper00044638:rsks-1(ok1255)_rep3 WBPaper00044638:rsks-1(ok1255)_rep4 WBPaper00044638:rsks-1(ok1255)_rep6 WBPaper00044638:rsks-1(ok1255)_rep7 WBPaper00044638:rsks-1(ok1255)_rep8 WBPaper00044638:rsks-1(ok1255)_rep9 WBPaper00044638:rsks-1(ok1255)_rep10 WBPaper00044638:daf-2(e1370)_rep1 WBPaper00044638:daf-2(e1370)_rep2 WBPaper00044638:daf-2(e1370)_rep3 WBPaper00044638:daf-2(e1370)_rep5 WBPaper00044638:daf-2(e1370)_rep6 WBPaper00044638:daf-2(e1370)_rep7 WBPaper00044638:daf-2(e1370)_rep8 WBPaper00044638:daf-2(e1370)_rep9 WBPaper00044638:daf-2(e1370)_rep10 WBPaper00044638:daf-2(e1370)rsks-1(ok1255)_rep1 WBPaper00044638:daf-2(e1370)rsks-1(ok1255)_rep2 WBPaper00044638:daf-2(e1370)rsks-1(ok1255)_rep3 WBPaper00044638:daf-2(e1370)rsks-1(ok1255)_rep4 WBPaper00044638:daf-2(e1370)rsks-1(ok1255)_rep5 WBPaper00044638:daf-2(e1370)rsks-1(ok1255)_rep6 WBPaper00044638:daf-2(e1370)rsks-1(ok1255)_rep7 WBPaper00044638:daf-2(e1370)rsks-1(ok1255)_rep8 WBPaper00044638:daf-2(e1370)rsks-1(ok1255)_rep9 WBPaper00044638:daf-2(e1370)rsks-1(ok1255)_rep10 WBPaper00044638:daf-16(mgDf47);daf-2(e1370)rsks-1(ok1255)_rep1 WBPaper00044638:daf-16(mgDf47);daf-2(e1370)rsks-1(ok1255)_rep2 WBPaper00044638:daf-16(mgDf47);daf-2(e1370)rsks-1(ok1255)_rep3 WBPaper00044638:daf-16(mgDf47);daf-2(e1370)rsks-1(ok1255)_rep4 WBPaper00044638:daf-16(mgDf47);daf-2(e1370)rsks-1(ok1255)_rep5 WBPaper00044638:daf-16(mgDf47);daf-2(e1370)rsks-1(ok1255)_rep6 WBPaper00044638:daf-16(mgDf47);daf-2(e1370)rsks-1(ok1255)_rep7 WBPaper00044638:daf-16(mgDf47);daf-2(e1370)rsks-1(ok1255)_rep8 WBPaper00044638:daf-16(mgDf47);daf-2(e1370)rsks-1(ok1255)_rep9 WBPaper00044638:daf-16(mgDf47);daf-2(e1370)rsks-1(ok1255)_rep10.
Tags	Method: microarray, Species: Caenorhabditis elegans, Topic: aging