| Citation | Wong D, Bazopoulou D, Pujol N, Tavernarakis N, Ewbank JJ. Genome-wide investigation reveals pathogen-specific and shared signatures in the response of Caenorhabditis elegans to infection. Genome Biol, 2007. |
| PubMed ID | 17875205 |
| Short Description | Genome-wide investigation reveals pathogen-specific and shared signatures in the response of Caenorhabditis elegans to infection. GEO Record: N.A. Platform: N.A. Download gene-centric, log2 transformed data: WBPaper00030985.ce.mr.csv |
| # of Conditions | 34 |
Full Description
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ABSTRACT: BACKGROUND: There are striking similarities between the innate immune systems of invertebrates and vertebrates. Caenorhabditis elegans is increasingly used as a model for the study of innate immunity. Evidence is accumulating that C. elegans mounts distinct responses to different pathogens, but the true extent of this specificity is unclear. Here, we employ direct comparative genomic analyses to explore the nature of the host immune response. RESULTS: Using whole-genome microarrays representing 20334 genes, we analysed the transcriptional response of C. elegans to four bacterial pathogens. Different bacteria provoke pathogen-specific signatures within the host, involving differential regulation of 3.5-5% of all genes. These include genes that encode potential pathogen-recognition and antimicrobial proteins. Additionally, variance analysis revealed a robust pathogen-shared signature, involving 22 genes associated with proteolysis, cell death and stress responses. The expression of these genes, including those that mediate necrosis, is similarly altered following infection with three bacterial pathogens. We show that necrosis aggravates pathogenesis and accelerates the death of the host. CONCLUSION: Our results suggest that in C. elegans, different infections trigger both specific and pathogen-shared responses involving immune defence genes. The pathogen-shared response involves necrotic cell death, which has been associated with infection in humans. Our results are the first indication that necrosis is important for disease susceptibility in C. elegans. This opens the way for detailed study of the means by which certain bacteria exploit conserved elements of host cell death machinery to increase their effective virulence. Experimental Details: WBPaper00030985:Aeromonas_hydrophila_rep1 WBPaper00030985:Aeromonas_hydrophila_rep2 WBPaper00030985:Aeromonas_hydrophila_rep3 WBPaper00030985:Aeromonas_hydrophila_rep4 WBPaper00030985:Aeromonas_hydrophila_rep5 WBPaper00030985:Aeromonas_hydrophila_rep6 WBPaper00030985:Serratia_marcesens_rep1 WBPaper00030985:Serratia_marcesens_rep2 WBPaper00030985:Serratia_marcesens_rep3 WBPaper00030985:Serratia_marcesens_rep4 WBPaper00030985:Serratia_marcesens_rep5 WBPaper00030985:Serratia_marcesens_rep6 WBPaper00030985:Serratia_marcesens_rep7 WBPaper00030985:Serratia_marcesens_rep8 WBPaper00030985:Serratia_marcesens_rep9 WBPaper00030985:Serratia_marcesens_rep10 WBPaper00030985:Enterococcus_faecalis_rep1 WBPaper00030985:Enterococcus_faecalis_rep2 WBPaper00030985:Enterococcus_faecalis_rep3 WBPaper00030985:Enterococcus_faecalis_rep4 WBPaper00030985:Enterococcus_faecalis_rep5 WBPaper00030985:Enterococcus_faecalis_rep6 WBPaper00030985:Erwinia_carotovora_rep1 WBPaper00030985:Erwinia_carotovora_rep2 WBPaper00030985:Erwinia_carotovora_rep3 WBPaper00030985:Erwinia_carotovora_rep4 WBPaper00030985:Erwinia_carotovora_rep5 WBPaper00030985:Erwinia_carotovora_rep6 WBPaper00030985:Photorhabdus_luminescens_rep1 WBPaper00030985:Photorhabdus_luminescens_rep2 WBPaper00030985:Photorhabdus_luminescens_rep3 WBPaper00030985:Photorhabdus_luminescens_rep4 WBPaper00030985:Photorhabdus_luminescens_rep5 WBPaper00030985:Photorhabdus_luminescens_rep6. |
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