| Citation | Spike CA, Bader J, Reinke V, Strome S. DEPS-1 promotes P-granule assembly and RNA interference in C. elegans germ cells. Development, 2008. |
| PubMed ID | 18234720 |
| Short Description | DEPS-1 promotes P-granule assembly and RNA interference in C. elegans germ cells. GEO Record: GSE9993 Platform: GPL3860 Download gene-centric, log2 transformed data: WBPaper00031477.ce.mr.csv |
| # of Conditions | 4 |
Full Description
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P granules are germ-cell-specific cytoplasmic structures containing RNA and protein, and required for proper germ cell development in C. elegans. PGL-1 and GLH-1 were previously identified as critical components of P granules. We have identified a new P-granule-associated protein, DEPS-1, the loss of which disrupts P-granule structure and function. DEPS-1 is required for the proper localization of PGL-1 to P granules, the accumulation of glh-1 mRNA and protein, and germ cell proliferation and fertility at elevated temperatures. In addition, DEPS-1 is required for RNA interference (RNAi) of germline-expressed genes, possibly because DEPS-1 promotes the accumulation of RDE-4, a dsRNA-binding protein required for RNAi. A genome wide analysis of gene expression in deps-1 mutant germ lines identified additional targets of DEPS-1 regulation, many of which are also regulated by the RNAi factor RDE-3. Our studies suggest that DEPS-1 is a key component of the P-granule assembly pathway and that its roles include promoting accumulation of some mRNAs, such as glh-1 and rde-4, and reducing accumulation of other mRNAs, perhaps by collaborating with RDE-3 to generate endogenous short interfering RNAs (endo-siRNAs). Experimental Details: WBPaper00031477:deps-1_vs_N2_1 WBPaper00031477:deps-1_vs_N2_2 WBPaper00031477:N2_vs_deps-1_1 WBPaper00031477:N2_vs_deps-1_2. |
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