SPELL - Nematode

Citation	Ziegler K, Kurz CL, Cypowyj S, Couillault C, Pophillat M, Pujol N, Ewbank JJ. Antifungal innate immunity in C. elegans: PKCdelta links G protein signaling and a conserved p38 MAPK cascade. Cell Host Microbe, 2009.
PubMed ID	19380113
Short Description	Antifungal innate immunity in C. elegans: PKCdelta links G protein signaling and a conserved p38 MAPK cascade. GEO Record: N.A. Platform: N.A. Download gene-centric, log2 transformed data: WBPaper00033094.ce.mr.csv
# of Conditions	8
Full Description	Like other multicellular organisms, the model nematode C. elegans responds to infection by inducing the expression of defense genes. Among the genes upregulated in response to a natural fungal pathogen is nlp-29, encoding an antimicrobial peptide. In a screen for mutants that fail to express nlp-29 following fungal infection, we isolated alleles of tpa-1, homologous to the mammalian protein kinase C (PKC) delta. Through epistasis analyses, we demonstrate that C. elegans PKC acts through the p38 MAPK pathway to regulate nlp-29. This involves G protein signaling and specific C-type phospholipases acting upstream of PKCdelta. Unexpectedly and unlike in mammals, tpa-1 does not act via D-type protein kinases, but another C. elegans PKC gene, pkc-3, functions nonredundantly with tpa-1 to control nlp-29 expression. Finally, the tribbles-like kinase nipi-3 acts upstream of PKCdelta in this antifungal immune signaling cascade. These findings greatly expand our understanding of the pathways involved in C. elegans innate immunity. Experimental Details: WBPaper00033094:tpa-1_DConiospora_vs_tpa-1_control_rep1 WBPaper00033094:tpa-1_DConiospora_vs_tpa-1_control_rep2 WBPaper00033094:tpa-1_DConiospora_vs_tpa-1_control_rep3 WBPaper00033094:tpa-1_DConiospora_vs_tpa-1_control_rep4 WBPaper00033094:WT_DConiospora_vs_WT_control_rep1 WBPaper00033094:WT_DConiospora_vs_WT_control_rep2 WBPaper00033094:WT_DConiospora_vs_WT_control_rep3 WBPaper00033094:WT_DConiospora_vs_WT_control_rep4.
Tags	Method: microarray, Species: Caenorhabditis elegans, Topic: innate immune response