SPELL - Nematode

Citation	Katz DJ, Edwards TM, Reinke V, Kelly WG. A C. elegans LSD1 demethylase contributes to germline immortality by reprogramming epigenetic memory. Cell, 2009.
PubMed ID	19379696
Short Description	A C. elegans LSD1 demethylase contributes to germline immortality by reprogramming epigenetic memory. GEO Record: GSE14432 Platform: GPL5859 Download gene-centric, log2 transformed data: WBPaper00033101.ce.mr.csv
# of Conditions	12
Full Description	Epigenetic information undergoes extensive reprogramming in the germline between generations. This reprogramming may be essential to establish a developmental ground state in the zygote. We show that mutants in spr-5, the Caenorhabditis elegans ortholog of the H3K4me2 demethylase LSD1/KDM1, exhibit progressive sterility over many generations. This sterility correlates with the misregulation of spermatogenesis-expressed genes and transgenerational accumulation of the histone modification dimethylation of histone H3 on lysine 4 (H3K4me2). This suggests that H3K4me2 can serve as a stable epigenetic memory, and that erasure of H3K4me2 by LSD/KDM1 in the germline prevents the inappropriate transmission of this epigenetic memory from one generation to the next. Thus, our results provide direct mechanistic insights into the processes that are required for epigenetic reprogramming between generations. Experimental Details: WBPaper00033101:f1_v_f13 WBPaper00033101:f1_v_f26 WBPaper00033101:f13_v_f1 WBPaper00033101:f1_v_N2 WBPaper00033101:f13_v_f26 WBPaper00033101:f13_v_N2 WBPaper00033101:f26_v_f1 WBPaper00033101:f26_v_f13 WBPaper00033101:f26_v_N2 WBPaper00033101:N2_v_f1 WBPaper00033101:N2_v_f13 WBPaper00033101:N2_v_f26.
Tags	Method: microarray, Species: Caenorhabditis elegans, Topic: unclassified