| Citation | Rogers AN, Chen D, McColl G, Czerwieniec G, Felkey K, Gibson BW, Hubbard A, Melov S, Lithgow GJ, Kapahi P. Life span extension via eIF4G inhibition is mediated by posttranscriptional remodeling of stress response gene expression in C. elegans. Cell Metab, 2011. |
| PubMed ID | 21723504 |
| Short Description | Life span extension via eIF4G inhibition is mediated by posttranscriptional remodeling of stress response gene expression in C. elegans. GEO Record: GSE28665 Platform: GPL9458 Download gene-centric, log2 transformed data: WBPaper00039835.ce.mr.csv |
| # of Conditions | 24 |
Full Description
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Reducing protein synthesis slows growth and development but can increase adult life span. We demonstrate that knockdown of eukaryotic translation initiation factor 4G (eIF4G), which is downregulated during starvation and dauer state, results in differential translation of genes important for growth and longevity in C.elegans. Genome-wide mRNA translation state analysis showed that inhibition of IFG-1, the C.elegans ortholog of eIF4G, results in a relative increase in ribosomal loading and translation of stress response genes. Some of these genes are required for life span extension when IFG-1 is inhibited. Furthermore, enhanced ribosomal loading of certain mRNAs upon IFG-1 inhibition was correlated with increased mRNA length. This association was supported by changes in the proteome assayed via quantitative mass spectrometry. Our results suggest that IFG-1 mediates the antagonistic effects on growth and somatic maintenance by regulating mRNA translation of particular mRNAs based, in part, on transcript length. Experimental Details: WBPaper00039835:N2_control_Fraction1_group1 WBPaper00039835:N2_control_Fraction2_group1 WBPaper00039835:N2_control_Fraction3_group1 WBPaper00039835:N2_control_Fraction1_group2 WBPaper00039835:N2_control_Fraction2_group2 WBPaper00039835:N2_control_Fraction3_group2 WBPaper00039835:N2_control_Fraction1_group3 WBPaper00039835:N2_control_Fraction2_group3 WBPaper00039835:N2_control_Fraction3_group3 WBPaper00039835:N2_control_Fraction1_group4 WBPaper00039835:N2_control_Fraction2_group4 WBPaper00039835:N2_control_Fraction3_group4 WBPaper00039835:N2_ifg-1_Fraction1_group1 WBPaper00039835:N2_ifg-1_Fraction2_group1 WBPaper00039835:N2_ifg-1_Fraction3_group1 WBPaper00039835:N2_ifg-1_Fraction1_group2 WBPaper00039835:N2_ifg-1_Fraction2_group2 WBPaper00039835:N2_ifg-1_Fraction3_group2 WBPaper00039835:N2_ifg-1_Fraction1_group3 WBPaper00039835:N2_ifg-1_Fraction2_group3 WBPaper00039835:N2_ifg-1_Fraction3_group3 WBPaper00039835:N2_ifg-1_Fraction1_group4 WBPaper00039835:N2_ifg-1_Fraction2_group4 WBPaper00039835:N2_ifg-1_Fraction3_group4. |
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