| Citation | Kudlow BA, Zhang L, Han M. Systematic analysis of tissue-restricted miRISCs reveals a broad role for microRNAs in suppressing basal activity of the C. elegans pathogen response. Mol Cell, 2012. |
| PubMed ID | 22503424 |
| Short Description | Systematic analysis of tissue-restricted miRISCs reveals a broad role for microRNAs in suppressing basal activity of the C. elegans pathogen response. GEO Record: GSE36419 Platform: GPL5883 Download gene-centric, log2 transformed data: WBPaper00040985.ce.mr.csv |
| # of Conditions | 15 |
Full Description
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Gene regulation by microRNAs (miRNAs) under specific physiological conditions often involves complex interactions between multiple miRNAs and a large number of their targets, as well as coordination with other regulatory mechanisms, limiting the effectiveness of classical genetic methods to identify miRNA functions. We took a systematic approach to analyze the miRNA-induced silencing complex (miRISC) in individual tissues of C. elegans and found that mRNAs encoded by pathogen-responsive genes were dramatically overrepresented in the intestinal miRISC, and that multiple miRNAs accumulated in the intestinal miRISCs upon infection. Inactivation of the miRISC or ablation of miRNAs from multiple families resulted in overexpression of several pathogen-responsive genes under basal conditions and, surprisingly, enhanced worm survival on pathogenic Pseudomonas aeruginosa. These results indicate that much of the miRNA activity in the gut is dedicated to attenuating the activity of the pathogen-response system, uncovering a complex physiological function of the miRNA network. Experimental Details: WBPaper00040985:Asynch-Intestine_IP_vs_Total-Rep1 WBPaper00040985:Asynch-Intestine_IP_vs_Total-Rep2 WBPaper00040985:Asynch-Intestine_IP_vs_Total-Rep3 WBPaper00040985:Asynch-Intestine_IP_vs_Total-Rep4 WBPaper00040985:Asynch-Muscle_IP_vs_Total-Rep1 WBPaper00040985:Asynch-Muscle_IP_vs_Total-Rep2 WBPaper00040985:Asynch-Muscle_IP_vs_Total-Rep3 WBPaper00040985:Asynch-Muscle_IP_vs_Total-Rep4 WBPaper00040985:L4-Intestine_IP_vs_Total-Rep1 WBPaper00040985:L4-Intestine_IP_vs_Total-Rep2 WBPaper00040985:L4-Intestine_IP_vs_Total-Rep3 WBPaper00040985:L4-Intestine_IP_vs_Total-Rep4 WBPaper00040985:L4-Intestine_IP_vs_Total-Rep5 WBPaper00040985:GFP_IP_vs_Total-Rep1 WBPaper00040985:GFP_IP_vs_Total-Rep2. |
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