| Citation | Block DH, Twumasi-Boateng K, Kang HS, Carlisle JA, Hanganu A, Lai TY, Shapira M. The Developmental Intestinal Regulator ELT-2 Controls p38-Dependent Immune Responses in Adult C. elegans. PLoS Genet, 2015. |
| PubMed ID | 26016853 |
| Short Description | The Developmental Intestinal Regulator ELT-2 Controls p38-Dependent Immune Responses in Adult C. elegans. GEO Record: GSE63846 Platform: GPL9458 Download gene-centric, log2 transformed data: WBPaper00046858.ce.mr.csv |
| # of Conditions | 14 |
Full Description
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GATA transcription factors play critical roles in cellular differentiation and development. However, their roles in mature tissues are less understood. In C. elegans larvae, the transcription factor ELT-2 regulates terminal differentiation of the intestine. It is also expressed in the adult intestine, where it was suggested to maintain intestinal structure and function, and where it was additionally shown to contribute to infection resistance. To study the function of elt-2 in adults we characterized elt-2-dependent gene expression following its knock-down specifically in adults. Microarray analysis identified two ELT-2-regulated gene subsets: one, enriched for hydrolytic enzymes, pointed at regulation of constitutive digestive functions as a dominant role of adult elt-2; the second was enriched for immune genes that are induced in response to Pseudomonas aeruginosa infection. Focusing on the latter, we used genetic analyses coupled to survival assays and quantitative RT-PCR to interrogate the mechanism(s) through which elt-2 contributes to immunity. We show that elt-2 controls p38-dependent gene induction, cooperating with two p38-activated transcription factors, ATF-7 and SKN-1. This demonstrates a mechanism through which the constitutively nuclear elt-2 can impact induced responses, and play a dominant role in C. elegans immunity. Experimental Details: WBPaper00046858:control-RNAi_OP50_hand-picked_rep1 WBPaper00046858:control-RNAi_OP50_worm-sorter_rep2 WBPaper00046858:control-RNAi_OP50_worm-sorter_rep3 WBPaper00046858:control-RNAi_PA14-non-colonized_hand-picked_rep1 WBPaper00046858:control-RNAi_PA14-non-colonized_worm-sorter_rep2 WBPaper00046858:control-RNAi_PA14-non-colonized_worm-sorter_rep3 WBPaper00046858:control-RNAi_PA14-colonized_hand-picked_rep1 WBPaper00046858:control-RNAi_PA14-colonized_worm-sorter_rep2 WBPaper00046858:control-RNAi_PA14-colonized_worm-sorter_rep3 WBPaper00046858:elt-2(RNAi)_OP50_hand-picked_rep1 WBPaper00046858:elt-2(RNAi)_OP50_wormsorter_rep2 WBPaper00046858:elt-2(RNAi)_PA14-non-colonized_hand-picked_rep1 WBPaper00046858:elt-2(RNAi)_PA14-non-colonized_worm-sorter_rep2 WBPaper00046858:elt-2(RNAi)_PA14-colonized_hand-picked_rep1. |
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