| Citation | Zhang Z, Liu L, Twumasi-Boateng K, Block DH, Shapira M. FOS-1 functions as a transcriptional activator downstream of the C. elegans JNK homolog KGB-1. Cell Signal, 2016. |
| PubMed ID | 27864060 |
| Short Description | FOS-1 functions as a transcriptional activator downstream of the C. elegans JNK homolog KGB-1. GEO Record: GSE82238 Platform: GPL9458 Download gene-centric, log2 transformed data: WBPaper00050448.ce.mr.csv |
| # of Conditions | 33 |
Full Description
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JNK proteins are conserved stress-activated MAP kinases. In C. elegans, the JNK-homolog KGB-1 plays essential roles in protection from heavy metals and protein folding stress. However, the contributions of KGB-1 are age-dependent, providing protection in larvae, but reducing stress resistance and shortening lifespan in adults. Attenuation of DAF-16 was linked to the detrimental contributions of KGB-1 in adults, but its involvement in KGB-1-dependent protection in larvae remains unclear. To characterize age-dependent contributions of KGB-1, we used microarray analysis to measure gene expression following KGB-1 activation either in developing larvae or in adults, achieved by knocking down its negative phosphatase regulator vhp-1. This revealed a robust KGB-1 regulon, most of which consisting of genes induced following KGB-1 activation regardless of age; a smaller number of genes was regulated in an age-dependent manner. We found that the bZIP transcription factor FOS-1 was essential for age-invariant KGB-1-dependent gene induction, but not for age-dependent expression. The latter was more affected by DAF-16, which was further found to be required for KGB-1-dependent cadmium resistance in larvae. Our results identify FOS-1 as a transcriptional activator mediating age-invariant contributions of KGB-1, including a regulatory loop of KGB-1 signaling, but also stress the importance of DAF-16 as a mediator of age-dependent contributions. Experimental Details: WBPaper00050448:N2_control(RNAi)_development_rep1 WBPaper00050448:N2_control(RNAi)_development_rep2 WBPaper00050448:N2_control(RNAi)_development_rep3 WBPaper00050448:N2_vhp-1(RNAi)_development_rep1 WBPaper00050448:N2_vhp-1(RNAi)_development_rep2 WBPaper00050448:N2_vhp-1(RNAi)_development_rep3 WBPaper00050448:N2_control(RNAi)_adult_rep1 WBPaper00050448:N2_control(RNAi)_adult_rep2 WBPaper00050448:N2_control(RNAi)_adult_rep3 WBPaper00050448:N2_vhp-1(RNAi)_adult_rep1 WBPaper00050448:N2_vhp-1(RNAi)_adult_rep2 WBPaper00050448:N2_vhp-1(RNAi)_adult_rep3 WBPaper00050448:kgb-1(km21)_control(RNAi)_development_rep1 WBPaper00050448:kgb-1(km21)_control(RNAi)_development_rep2 WBPaper00050448:kgb-1(km21)_control(RNAi)_development_rep3 WBPaper00050448:kgb-1(km21)_vhp-1(RNAi)_development_rep1 WBPaper00050448:kgb-1(km21)_vhp-1(RNAi)_development_rep2 WBPaper00050448:kgb-1(km21)_vhp-1(RNAi)_development_rep3 WBPaper00050448:kgb-1(km21)_control(RNAi)_adult_rep1 WBPaper00050448:kgb-1(km21)_control(RNAi)_adult_rep2 WBPaper00050448:kgb-1(km21)_control(RNAi)_adult_rep3 WBPaper00050448:kgb-1(km21)_control(RNAi)_adult_rep4 WBPaper00050448:kgb-1(km21)_vhp-1(RNAi)_adult_rep1 WBPaper00050448:kgb-1(km21)_vhp-1(RNAi)_adult_rep2 WBPaper00050448:kgb-1(km21)_vhp-1(RNAi)_adult_rep3 WBPaper00050448:daf-16(mu86);pmk-1(km25)_control(RNAi)_development_rep1 WBPaper00050448:daf-16(mu86);pmk-1(km25)_control(RNAi)_development_rep2 WBPaper00050448:daf-16(mu86);pmk-1(km25)_vhp-1(RNAi)_development_rep1 WBPaper00050448:daf-16(mu86);pmk-1(km25)_vhp-1(RNAi)_development_rep2 WBPaper00050448:daf-16(mu86);pmk-1(km25)_control(RNAi)_adult_rep1 WBPaper00050448:daf-16(mu86);pmk-1(km25)_control(RNAi)_adult_rep2 WBPaper00050448:daf-16(mu86);pmk-1(km25)_vhp-1(RNAi)_adult_rep1 WBPaper00050448:daf-16(mu86);pmk-1(km25)_vhp-1(RNAi)_adult_rep2. |
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