| Citation | Rhoads TW, Prasad A, Kwiecien NW, Merrill AE, Zawack K, Westphall MS, Schroeder FC, Kimble J, Coon JJ. NeuCode Labeling in Nematodes: Proteomic and Phosphoproteomic Impact of Ascaroside Treatment in Caenorhabditis elegans. Mol Cell Proteomics, 2015. |
| PubMed ID | 26392051 |
| Short Description | NeuCode Labeling in Nematodes: Proteomic and Phosphoproteomic Impact of Ascaroside Treatment in Caenorhabditis elegans. GEO Record: N.A. Platform: N.A. Download gene-centric, log2 transformed data: WBPaper00048573.ce.ms.csv |
| # of Conditions | 9 |
Full Description
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The nematode Caenorhabditis elegans is an important model organism for biomedical research. We previously described NeuCode stable isotope labeling by amino acids in cell culture (SILAC), a method for accurate proteome quantification with potential for multiplexing beyond the limits of traditional stable isotope labeling by amino acids in cell culture. Here we apply NeuCode SILAC to profile the proteomic and phosphoproteomic response of C. elegans to two potent members of the ascaroside family of nematode pheromones. By consuming labeled E. coli as part of their diet, C. elegans nematodes quickly and easily incorporate the NeuCode heavy lysine isotopologues by the young adult stage. Using this approach, we report, at high confidence, one of the largest proteomic and phosphoproteomic data sets to date in C. elegans: 6596 proteins at a false discovery rate 1% and 6620 phosphorylation isoforms with localization probability 75%. Our data reveal a post-translational signature of pheromone sensing that includes many conserved proteins implicated in longevity and response to stress. Experimental Details: MassSpec_WBPaper00048573:Ascaroside-2_rep1 MassSpec_WBPaper00048573:Ascaroside-2_rep2 MassSpec_WBPaper00048573:Ascaroside-2_rep3 MassSpec_WBPaper00048573:Ascaroside-5_rep1 MassSpec_WBPaper00048573:Ascaroside-5_rep2 MassSpec_WBPaper00048573:Ascaroside-5_rep3 MassSpec_WBPaper00048573:Control_rep1 MassSpec_WBPaper00048573:Control_rep2 MassSpec_WBPaper00048573:Control_rep3. |
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