SPELL - Nematode

Citation	Sharifi S, Chaudhari P, Martirosyan A, Eberhardt AO, Witt F, Gollowitzer A, Lange L, Woitzat Y, Okoli EM, Li H, Rahnis N, Kirkpatrick J, Werz O, Ori A, Koeberle A, Bierhoff H, Ermolaeva M. Reducing the metabolic burden of rRNA synthesis promotes healthy longevity in Caenorhabditis elegans. Nat Commun, 2024.
PubMed ID	38402241
Short Description	Reducing the metabolic burden of rRNA synthesis promotes healthy longevity in Caenorhabditis elegans. GEO Record: N.A. Platform: N.A. Download gene-centric, log2 transformed data: WBPaper00066531.ce.ms.csv
# of Conditions	45
Full Description	Ribosome biogenesis is initiated by RNA polymerase I (Pol I)-mediated synthesis of pre-ribosomal RNA (pre-rRNA). Pol I activity was previously linked to longevity, but the underlying mechanisms were not studied beyond effects on nucleolar structure and protein translation. Here we use multi-omics and functional tests to show that curtailment of Pol I activity remodels the lipidome and preserves mitochondrial function to promote longevity in Caenorhabditis elegans. Reduced pre-rRNA synthesis improves energy homeostasis and metabolic plasticity also in human primary cells. Conversely, the enhancement of pre-rRNA synthesis boosts growth and neuromuscular performance of young nematodes at the cost of accelerated metabolic decline, mitochondrial stress and premature aging. Moreover, restriction of Pol I activity extends lifespan more potently than direct repression of protein synthesis, and confers geroprotection even when initiated late in life, showcasing this intervention as an effective longevity and metabolic health treatment not limited by aging. Experimental Details: MassSpec_WBPaper00066531:EVD2_rep1_1 MassSpec_WBPaper00066531:EVD2_rep2_2 MassSpec_WBPaper00066531:EVD2_rep3_3 MassSpec_WBPaper00066531:EVD2_rep4_4 MassSpec_WBPaper00066531:EVD2_rep5_5 MassSpec_WBPaper00066531:EVD6_rep1_6 MassSpec_WBPaper00066531:EVD6_rep2_7 MassSpec_WBPaper00066531:EVD6_rep3_8 MassSpec_WBPaper00066531:EVD6_rep_4_9 MassSpec_WBPaper00066531:EVD6_rep5_10 MassSpec_WBPaper00066531:EVD12_rep2_12 MassSpec_WBPaper00066531:EVD12_rep1_11 MassSpec_WBPaper00066531:EVD12_rep3_13 MassSpec_WBPaper00066531:EVD12_rep4_14 MassSpec_WBPaper00066531:EVD12_rep5_15 MassSpec_WBPaper00066531:C36D2_rep1_16 MassSpec_WBPaper00066531:C36D2_rep2_17 MassSpec_WBPaper00066531:C36D2_rep3_18 MassSpec_WBPaper00066531:C36D2_rep4_19 MassSpec_WBPaper00066531:C36D2_rep5_20 MassSpec_WBPaper00066531:C36D6_rep1_21 MassSpec_WBPaper00066531:C36D6_rep2_22 MassSpec_WBPaper00066531:C36D6_rep3_23 MassSpec_WBPaper00066531:C36D6_rep4_24 MassSpec_WBPaper00066531:C36D6_rep5_25 MassSpec_WBPaper00066531:C36D12_rep1_26 MassSpec_WBPaper00066531:C36D12_rep2_27 MassSpec_WBPaper00066531:C36D12_rep3_28 MassSpec_WBPaper00066531:C36D12_rep4_29 MassSpec_WBPaper00066531:C36D12_rep5_30 MassSpec_WBPaper00066531:NCL1D2_rep1_31 MassSpec_WBPaper00066531:NCL1D2_rep2_32 MassSpec_WBPaper00066531:NCL1D2_rep_3_33 MassSpec_WBPaper00066531:NCL1D2_rep4_34 MassSpec_WBPaper00066531:NCL1D2_rep5_35 MassSpec_WBPaper00066531:NCL1D6_rep1_36 MassSpec_WBPaper00066531:NCL1D6_rep2_37 MassSpec_WBPaper00066531:NCL1D6_rep3_38 MassSpec_WBPaper00066531:NCL1D6_rep4_39 MassSpec_WBPaper00066531:NCL1D6_rep5_40 MassSpec_WBPaper00066531:NCL1D12_rep1_41 MassSpec_WBPaper00066531:NCL1D12_rep2_42 MassSpec_WBPaper00066531:NCL1D12_rep3_43 MassSpec_WBPaper00066531:NCL1D12_rep4_44 MassSpec_WBPaper00066531:NCL1D12_rep5_45.
Tags	Method: proteomics, Species: Caenorhabditis elegans, Topic: aging