| Citation | Schwarz EM, Kato M, Sternberg PW. Functional transcriptomics of a migrating cell in Caenorhabditis elegans. Proc Natl Acad Sci U S A, 2012. |
| PubMed ID | 22991463 |
| Short Description | Functional transcriptomics of a migrating cell in Caenorhabditis elegans. GEO Record: N.A. Platform: N.A. Download gene-centric, log2 transformed data: WBPaper00041549.ce.rs.csv |
| # of Conditions | 20 |
Full Description
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In both metazoan development and metastatic cancer, migrating cells must carry out a detailed, complex program of sensing cues, binding substrates, and moving their cytoskeletons. The linker cell in Caenorhabditis elegans males undergoes a stereotyped migration that guides gonad organogenesis, occurs with precise timing, and requires the nuclear hormone receptor NHR-67. To better understand how this occurs, we performed RNA-seq of individually staged and dissected linker cells, comparing transcriptomes from linker cells of third-stage (L3) larvae, fourth-stage (L4) larvae, and nhr-67-RNAi-treated L4 larvae. We observed expression of 8,000-10,000 genes in the linker cell, 22-25% of which were up- or down-regulated 20-fold during development by NHR-67. Of genes that we tested by RNAi, 22% (45 of 204) were required for normal shape and migration, suggesting that many NHR-67-dependent, linker cell-enriched genes play roles in this migration. One unexpected class of genes up-regulated by NHR-67 was tandem pore potassium channels, which are required for normal linker-cell migration. We also found phenotypes for genes with human orthologs but no previously described migratory function. Our results provide an extensive catalog of genes that act in a migrating cell, identify unique molecular functions involved in nematode cell migration, and suggest similar functions in humans. Experimental Details: RNASeq.elegans.WBStrain00000001.WBls:0000002.Hermaphrodite.WBbt:0007833.SRP015688.SRX185637 RNASeq.elegans.WBStrain00030950.WBls:0000038.Male.WBbt:0005062.SRP015688.SRX185638 RNASeq.elegans.WBStrain00000001.WBls:0000023.Hermaphrodite.WBbt:0007833.SRP015688.SRX185639 RNASeq.elegans.WBStrain00030950.WBls:0000035.Male.WBbt:0005062.SRP015688.SRX185660 RNASeq.elegans.WBStrain00030950.WBls:0000038.Male.WBbt:0005062.SRP015688.SRX185661 RNASeq.elegans.WBStrain00030950.WBls:0000038.Male.WBbt:0005062.SRP015688.SRX185662 RNASeq.elegans.WBStrain00030950.WBls:0000038.Male.WBbt:0005062.SRP015688.SRX185663 RNASeq.elegans.WBStrain00030950.WBls:0000038.Male.WBbt:0005062.SRP015688.SRX185664 RNASeq.elegans.WBStrain00030950.WBls:0000038.Male.WBbt:0005062.SRP015688.SRX185665 RNASeq.elegans.WBStrain00030950.WBls:0000038.Male.WBbt:0005062.SRP015688.SRX185666 RNASeq.elegans.WBStrain00030950.WBls:0000035.Male.WBbt:0005062.SRP015688.SRX185667 RNASeq.elegans.WBStrain00030950.WBls:0000035.Male.WBbt:0005062.SRP015688.SRX185668 RNASeq.elegans.WBStrain00030950.WBls:0000035.Male.WBbt:0005062.SRP015688.SRX185669 RNASeq.elegans.WBStrain00030950.WBls:0000035.Male.WBbt:0005062.SRP015688.SRX185670 RNASeq.elegans.WBStrain00030950.WBls:0000035.Male.WBbt:0005062.SRP015688.SRX185672 RNASeq.elegans.WBStrain00030950.WBls:0000038.Male.WBbt:0005062.SRP015688.SRX185674 RNASeq.elegans.WBStrain00030950.WBls:0000038.Male.WBbt:0005062.SRP015688.SRX185676 RNASeq.elegans.WBStrain00030950.WBls:0000038.Male.WBbt:0005062.SRP015688.SRX185678 RNASeq.elegans.WBStrain00030950.WBls:0000038.Male.WBbt:0005062.SRP015688.SRX185680 RNASeq.elegans.WBStrain00030950.WBls:0000038.Male.WBbt:0005062.SRP015688.SRX185681. |
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