SPELL - Nematode

Citation	Surya A, Bolton BM, Rothe R, Mejia-Trujillo R, Leonita A, Zhao Q, Arya A, Liu Y, Rangan R, Gorusu Y, Nguyen P, Cenik C, Sarinay Cenik E. Differential impacts of ribosomal protein haploinsufficiency on mitochondrial function. J Cell Biol, 2025.
PubMed ID	39786340
Short Description	Differential impacts of ribosomal protein haploinsufficiency on mitochondrial function. GEO Record: N.A. Platform: N.A. Download gene-centric, log2 transformed data: WBPaper00067708.ce.ms.csv
# of Conditions	21
Full Description	The interplay between ribosomal protein (RP) composition and mitochondrial function is essential for energy homeostasis. Balanced RP production optimizes protein synthesis while minimizing energy costs, but its impact on mitochondrial functionality remains unclear. Here, we investigated haploinsufficiency for RP genes (rps-10, rpl-5, rpl-33, and rps-23) in Caenorhabditis elegans and corresponding reductions in human lymphoblast cells. Significant mitochondrial morphological differences, upregulation of glutathione transferases, and SKN-1-dependent oxidative stress resistance were observed across mutants. Loss of a Datasingle rps-10 copy reduced mitochondrial activity, energy levels, and oxygen consumption, mirrored by similar reductions in mitochondrial activity and energy levels in lymphoblast cells with 50% lower RPS10 transcripts. Both systems exhibited altered translation efficiency (TE) of mitochondrial electron transport chain components, suggesting a conserved mechanism to adjust mitochondrial protein synthesis under ribosomal stress. Finally, mitochondrial membrane and cytosolic RPs showed significant RNA and TE covariation in lymphoblastoid cells, highlighting the interplay between protein synthesis machinery and mitochondrial energy production. Experimental Details: MassSpec_WBPaper00067708:N2_mc6_rep1 MassSpec_WBPaper00067708:N2_mc6_rep2 MassSpec_WBPaper00067708:N2_mc6_rep3 MassSpec_WBPaper00067708:rpl-5_mc6_rep1 MassSpec_WBPaper00067708:rpl-5_mc6_rep2 MassSpec_WBPaper00067708:rpl-5_mc6_rep3 MassSpec_WBPaper00067708:rpl-33_mc6_rep1 MassSpec_WBPaper00067708:rpl-33_mc6_rep2 MassSpec_WBPaper00067708:rpl-33_mc6_rep3 MassSpec_WBPaper00067708:N2_tmc20_rep1 MassSpec_WBPaper00067708:N2_tmc20_rep2 MassSpec_WBPaper00067708:N2_tmc20_rep3 MassSpec_WBPaper00067708:rps-10_tmc20_rep1 MassSpec_WBPaper00067708:rps-10_tmc20_rep2 MassSpec_WBPaper00067708:rps-10_tmc20_rep3 MassSpec_WBPaper00067708:N2_tmc5_rep1 MassSpec_WBPaper00067708:N2_tmc5_rep2 MassSpec_WBPaper00067708:N2_tmc5_rep3 MassSpec_WBPaper00067708:rps-23_tmc5_rep1 MassSpec_WBPaper00067708:rps-23_tmc5_rep2 MassSpec_WBPaper00067708:rps-23_tmc5_rep3.
Tags	Method: proteomics, Species: Caenorhabditis elegans, Topic: WT vs. mutant