| Citation | Wu P, Vandemeulebroucke L, Cai H, Braeckman BP. The Proprotein Convertase BLI-4 Is Required for Axenic Dietary Restriction Mediated Longevity in Caenorhabditis elegans. Aging Cell, 2025. |
| PubMed ID | 40200707 |
| Short Description | The Proprotein Convertase BLI-4 Is Required for Axenic Dietary Restriction Mediated Longevity in Caenorhabditis elegans. GEO Record: N.A. Platform: N.A. Download gene-centric, log2 transformed data: WBPaper00067997.ce.ms.csv |
| # of Conditions | 20 |
Full Description
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Dietary restriction (DR) is a well-established method for extending lifespan across various species, including C. elegans. Among the different DR regimens, axenic dietary restriction (ADR), in which worms are grown in a nutrient-rich sterile liquid medium, yields the most powerful lifespan extension. However, the molecular mechanisms underlying this longevity phenotype remain largely unexplored. Through a pilot screen of candidate genes, we identified the proprotein convertase BLI-4 as a crucial factor in neurons for modulating lifespan under ADR conditions. BLI-4's role appears to be specific to ADR, as it does not significantly impact longevity under other DR regimens. We further explored the involvement of different bli-4 isoforms and found that isoforms b, f, i and j redundantly contribute to the ADR-mediated lifespan extension, while the bli-4d isoform is mainly involved in development. Proteomics analysis revealed that the loss of BLI-4 function under ADR conditions specifically downregulates GOLG-2, involved in Golgi complex organization. This gene also partially mediates the longevity effects of BLI-4 under ADR conditions. Our findings highlight the importance of neuronal BLI-4 and its downstream targets in regulating lifespan extension induced by ADR in C. elegans. Experimental Details: MassSpec_WBPaper00067997:Empty-Vector_Axenic-Dietary-Restriction_rep1 MassSpec_WBPaper00067997:Empty-Vector_Axenic-Dietary-Restriction_rep2 MassSpec_WBPaper00067997:Empty-Vector_Axenic-Dietary-Restriction_rep3 MassSpec_WBPaper00067997:Empty-Vector_Axenic-Dietary-Restriction_rep4 MassSpec_WBPaper00067997:Empty-Vector_Axenic-Dietary-Restriction_rep5 MassSpec_WBPaper00067997:Empty-Vector_Fully-Fed_rep1 MassSpec_WBPaper00067997:Empty-Vector_Fully-Fed_rep2 MassSpec_WBPaper00067997:Empty-Vector_Fully-Fed_rep3 MassSpec_WBPaper00067997:Empty-Vector_Fully-Fed_rep4 MassSpec_WBPaper00067997:Empty-Vector_Fully-Fed_rep5 MassSpec_WBPaper00067997:bli-4(RNAi)_Axenic-Dietary-Restriction_rep1 MassSpec_WBPaper00067997:bli-4(RNAi)_Axenic-Dietary-Restriction_rep2 MassSpec_WBPaper00067997:bli-4(RNAi)_Axenic-Dietary-Restriction_rep3 MassSpec_WBPaper00067997:bli-4(RNAi)_Axenic-Dietary-Restriction_rep4 MassSpec_WBPaper00067997:bli-4(RNAi)_Axenic-Dietary-Restriction_rep5 MassSpec_WBPaper00067997:bli-4(RNAi)_Fully-Fed_rep1 MassSpec_WBPaper00067997:bli-4(RNAi)_Fully-Fed_rep2 MassSpec_WBPaper00067997:bli-4(RNAi)_Fully-Fed_rep3 MassSpec_WBPaper00067997:bli-4(RNAi)_Fully-Fed_rep4 MassSpec_WBPaper00067997:bli-4(RNAi)_Fully-Fed_rep5. |
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