| Citation | Riedel CG, Dowen RH, Lourenco GF, Kirienko NV, Heimbucher T, West JA, Bowman SK, Kingston RE, Dillin A, Asara JM, Ruvkun G. DAF-16 employs the chromatin remodeller SWI/SNF to promote stress resistance and longevity. Nat Cell Biol, 2013. |
| PubMed ID | 23604319 |
| Short Description | DAF-16 employs the chromatin remodeller SWI/SNF to promote stress resistance and longevity. GEO Record: N.A. Platform: N.A. Download gene-centric, log2 transformed data: WBPaper00042296.ce.rs.csv |
| # of Conditions | 8 |
Full Description
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Organisms are constantly challenged by stresses and privations and require adaptive responses for their survival. The forkhead boxO (FOXO) transcription factor DAF-16 (hereafter referred to as DAF-16/FOXO) is a central nexus in these responses, but despite its importance little is known about how it regulates its target genes. Proteomic identification of DAF-16/FOXO-binding partners in Caenorhabditis elegans and their subsequent functional evaluation by RNA interference revealed several candidate DAF-16/FOXO cofactors, most notably the chromatin remodeller SWI/SNF. DAF-16/FOXO and SWI/SNF form a complex and globally co-localize at DAF-16/FOXO target promoters. We show that specifically for gene activation, DAF-16/FOXO depends on SWI/SNF, facilitating SWI/SNF recruitment to target promoters, to activate transcription by presumed remodelling of local chromatin. For the animal, this translates into an essential role for SWI/SNF in DAF-16/FOXO-mediated processes, in particular dauer formation, stress resistance and the promotion of longevity. Thus, we give insight into the mechanisms of DAF-16/FOXO-mediated transcriptional regulation and establish a critical link between ATP-dependent chromatin remodelling and lifespan regulation. Experimental Details: RNASeq.elegans.WBStrain00004309.WBls:0000064.Hermaphrodite.WBbt:0007833.SRP017908.SRX216722 RNASeq.elegans.WBStrain00004309.WBls:0000064.Hermaphrodite.WBbt:0007833.SRP017908.SRX216723 RNASeq.elegans.WBStrain00007897.WBls:0000064.Hermaphrodite.WBbt:0007833.SRP017908.SRX216724 RNASeq.elegans.WBStrain00007897.WBls:0000064.Hermaphrodite.WBbt:0007833.SRP017908.SRX216725 RNASeq.elegans.WBStrain00007952.WBls:0000064.Hermaphrodite.WBbt:0007833.SRP017908.SRX216726 RNASeq.elegans.WBStrain00007952.WBls:0000064.Hermaphrodite.WBbt:0007833.SRP017908.SRX216727 RNASeq.elegans.WBStrain00000001.WBls:0000064.Hermaphrodite.WBbt:0007833.SRP017908.SRX218153 RNASeq.elegans.WBStrain00000001.WBls:0000064.Hermaphrodite.WBbt:0007833.SRP017908.SRX218190. |
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