| Citation | Grote A, Voronin D, Ding T, Twaddle A, Unnasch TR, Lustigman S, Ghedin E. Defining Brugia malayi and Wolbachia symbiosis by stage-specific dual RNA-seq. PLoS Negl Trop Dis, 2017. |
| PubMed ID | 28358880 |
| Short Description | Defining Brugia malayi and Wolbachia symbiosis by stage-specific dual RNA-seq. GEO Record: N.A. Platform: N.A. Download gene-centric, log2 transformed data: WBPaper00051007.bma.rs.csv |
| # of Conditions | 14 |
Full Description
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BACKGROUND: Filarial nematodes currently infect up to 54 million people worldwide, with millions more at risk for infection, representing the leading cause of disability in the developing world. Brugia malayi is one of the causative agents of lymphatic filariasis and remains the only human filarial parasite that can be maintained in small laboratory animals. Many filarial nematode species, including B. malayi, carry an obligate endosymbiont, the alpha-proteobacteria Wolbachia, which can be eliminated through antibiotic treatment. Elimination of the endosymbiont interferes with development, reproduction, and survival of the worms within the mamalian host, a clear indicator that the Wolbachia are crucial for survival of the parasite. Little is understood about the mechanism underlying this symbiosis. METHODOLOGY/ PRINCIPLE FINDINGS: To better understand the molecular interplay between these two organisms we profiled the transcriptomes of B. malayi and Wolbachia by dual RNA-seq across the life cycle of the parasite. This helped identify functional pathways involved in this essential symbiotic relationship provided by the co-expression of nematode and bacterial genes. We have identified significant stage-specific and gender-specific differential expression in Wolbachia during the nematode's development. For example, during female worm development we find that Wolbachia upregulate genes involved in ATP production and purine biosynthesis, as well as genes involved in the oxidative stress response. CONCLUSIONS/ SIGNIFICANCE: This global transcriptional analysis has highlighted specific pathways to which both Wolbachia and B. malayi contribute concurrently over the life cycle of the parasite, paving the way for the development of novel intervention strategies. Experimental Details: RNASeq.brugia.WBStrain00041073.WBls:0000083.female.WBbt:0007833.SRP090644.SRX2200117 RNASeq.brugia.WBStrain00041073.WBls:0000083.female.WBbt:0007833.SRP090644.SRX2200118 RNASeq.brugia.WBStrain00041073.WBls:0000083.male.WBbt:0007833.SRP090644.SRX2200119 RNASeq.brugia.WBStrain00041073.WBls:0000083.male.WBbt:0007833.SRP090644.SRX2200120 RNASeq.brugia.WBStrain00041073.WBls:0000081.Unknown.WBbt:0007833.SRP090644.SRX2200121 RNASeq.brugia.WBStrain00041073.WBls:0000081.Unknown.WBbt:0007833.SRP090644.SRX2200122 RNASeq.brugia.WBStrain00041073.WBls:0000083.male.WBbt:0007833.SRP090644.SRX2200123 RNASeq.brugia.WBStrain00041073.WBls:0000083.male.WBbt:0007833.SRP090644.SRX2200124 RNASeq.brugia.WBStrain00041073.WBls:0000083.female.WBbt:0007833.SRP090644.SRX2200125 RNASeq.brugia.WBStrain00041073.WBls:0000083.female.WBbt:0007833.SRP090644.SRX2200126 RNASeq.brugia.WBStrain00041073.WBls:0000083.male.WBbt:0007833.SRP090644.SRX2200127 RNASeq.brugia.WBStrain00041073.WBls:0000083.male.WBbt:0007833.SRP090644.SRX2200128 RNASeq.brugia.WBStrain00041073.WBls:0000083.female.WBbt:0007833.SRP090644.SRX2200129 RNASeq.brugia.WBStrain00041073.WBls:0000083.female.WBbt:0007833.SRP090644.SRX2200130. |
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