| Citation | Conine CC, Moresco JJ, Gu W, Shirayama M, Conte D, Yates JR, Mello CC. Argonautes promote male fertility and provide a paternal memory of germline gene expression in C. elegans. Cell, 2013. |
| PubMed ID | 24360276 |
| Short Description | Argonautes promote male fertility and provide a paternal memory of germline gene expression in C. elegans. GEO Record: GSE49672 Platform: GPL13776 Download gene-centric, log2 transformed data: WBPaper00044616.ce.rs.csv |
| # of Conditions | 4 |
Full Description
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During each life cycle, germ cells preserve and pass on both genetic and epigenetic information. In C.elegans, the ALG-3/4 Argonaute proteins are expressed during male gametogenesis and promote male fertility. Here, we show that the CSR-1 Argonaute functions with ALG-3/4 to positively regulate target genes required for spermiogenesis. Our findings suggest that ALG-3/4 functions during spermatogenesis to amplify a small RNA signal that represents an epigenetic memory of male-specific gene expression. CSR-1, which is abundant in mature sperm, appears to transmit this memory to offspring. Surprisingly, in addition to small RNAs targeting male-specific genes, we show that males also harbor an extensive repertoire of CSR-1 smallRNAs targeting oogenesis-specific mRNAs. Together, these findings suggest that C.elegans sperm transmit not only the genome but also epigenetic binary signals in the form of Argonaute/small RNA complexes that constitute a memory of gene expression in preceding generations. Experimental Details: RNASeq.elegans.WBStrain00000001.WBls:0000041.Male.WBbt:0005784.SRP028611.SRX333008 RNASeq.elegans.WBStrain00000001.WBls:0000041.Male.WBbt:0005784.SRP028611.SRX333009 RNASeq.elegans.WBStrain00040479.WBls:0000041.Male.WBbt:0005784.SRP028611.SRX333010 RNASeq.elegans.WBStrain00040479.WBls:0000041.Male.WBbt:0005784.SRP028611.SRX333011. |
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