| Citation | Nousch M, Yeroslaviz A, Habermann B, Eckmann CR. The cytoplasmic poly(A) polymerases GLD-2 and GLD-4 promote general gene expression via distinct mechanisms. Nucleic Acids Res, 2014. |
| PubMed ID | 25217583 |
| Short Description | The cytoplasmic poly(A) polymerases GLD-2 and GLD-4 promote general gene expression via distinct mechanisms. GEO Record: N.A. Platform: N.A. Download gene-centric, log2 transformed data: WBPaper00045729.ce.rs.csv |
| # of Conditions | 9 |
Full Description
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Post-transcriptional gene regulation mechanisms decide on cellular mRNA activities. Essential gatekeepers of post-transcriptional mRNA regulation are broadly conserved mRNA-modifying enzymes, such as cytoplasmic poly(A) polymerases (cytoPAPs). Although these non-canonical nucleotidyltransferases efficiently elongate mRNA poly(A) tails in artificial tethering assays, we still know little about their global impact on poly(A) metabolism and their individual molecular roles in promoting protein production in organisms. Here, we use the animal model Caenorhabditis elegans to investigate the global mechanisms of two germline-enriched cytoPAPs, GLD-2 and GLD-4, by combining polysome profiling with RNA sequencing. Our analyses suggest that GLD-2 activity mediates mRNA stability of many translationally repressed mRNAs. This correlates with a general shortening of long poly(A) tails in gld-2-compromised animals, suggesting that most if not all targets are stabilized via robust GLD-2-mediated polyadenylation. By contrast, only mild polyadenylation defects are found in gld-4-compromised animals and few mRNAs change in abundance. Interestingly, we detect a reduced number of polysomes in gld-4 mutants and GLD-4 protein co-sediments with polysomes, which together suggest that GLD-4 might stimulate or maintain translation directly. Our combined data show that distinct cytoPAPs employ different RNA-regulatory mechanisms to promote gene expression, offering new insights into translational activation of mRNAs. Experimental Details: RNASeq.elegans.WBStrain00000001.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP043990.SRX643418 RNASeq.elegans.WBStrain00000001.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP043990.SRX643419 RNASeq.elegans.WBStrain00000001.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP043990.SRX643420 RNASeq.elegans.WBStrain00000001.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP043990.SRX643421 RNASeq.elegans.WBStrain00000001.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP043990.SRX643422 RNASeq.elegans.WBStrain00000001.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP043990.SRX643423 RNASeq.elegans.WBStrain00000001.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP043990.SRX643424 RNASeq.elegans.WBStrain00000001.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP043990.SRX643425 RNASeq.elegans.WBStrain00000001.WBls:0000063.Hermaphrodite.WBbt:0007833.SRP043990.SRX643426. |
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