SPELL - Nematode

Citation	Dallaire A, Proulx S, Simard MJ, Lebel M. Expression profile of Caenorhabditis elegans mutant for the Werner syndrome gene ortholog reveals the impact of vitamin C on development to increase life span. BMC Genomics, 2014.
PubMed ID	25346348
Short Description	Expression profile of Caenorhabditis elegans mutant for the Werner syndrome gene ortholog reveals the impact of vitamin C on development to increase life span. GEO Record: N.A. Platform: N.A. Download gene-centric, log2 transformed data: WBPaper00045934.ce.rs.csv
# of Conditions	12
Full Description	BACKGROUND: Werner Syndrome (WS) is a rare disorder characterized by the premature onset of a number of age-related diseases. The gene responsible for WS encodes a DNA helicase/exonuclease protein believed to affect different aspects of transcription, replication, and DNA repair. Caenorhabditis elegans (C. elegans) with a nonfunctional wrn-1 DNA helicase ortholog also exhibits a shorter life span, which can be rescued by vitamin C. In this study, we analyzed the impact of a mutation in the wrn-1 gene and the dietary supplementation of vitamin C on the global mRNA expression of the whole C. elegans by the RNA-seq technology. RESULTS: Vitamin C increased the mean life span of the wrn-1(gk99) mutant and the N2 wild type strains at 25C. However, the alteration of gene expression by vitamin C is different between wrn-1(gk99) and wild type strains. We observed alteration in the expression of 1522 genes in wrn-1(gk99) worms compared to wild type animals. Such genes significantly affected the metabolism of lipid, cellular ketone, organic acid, and carboxylic acids. Vitamin C, in return, altered the expression of genes in wrn-1(gk99) worms involved in locomotion and anatomical structure development. Proteolysis was the only biological process significantly affected by vitamin C in wild type worms. CONCLUSIONS: Expression profiling of wrn-1(gk99) worms revealed a very different response to the addition of vitamin C compared to wild type worms. Finally, vitamin C extended the life span of wrn-1(gk99) animals by altering biological processes involved mainly in locomotion and anatomical structure development. Experimental Details: RNASeq.elegans.WBStrain00000001.WBls:0000038.Hermaphrodite.WBbt:0007833.SRP035479.SRX435689 RNASeq.elegans.WBStrain00000001.WBls:0000038.Hermaphrodite.WBbt:0007833.SRP035479.SRX435690 RNASeq.elegans.WBStrain00000001.WBls:0000038.Hermaphrodite.WBbt:0007833.SRP035479.SRX435691 RNASeq.elegans.WBStrain00000001.WBls:0000038.Hermaphrodite.WBbt:0007833.SRP035479.SRX435692 RNASeq.elegans.WBStrain00000001.WBls:0000038.Hermaphrodite.WBbt:0007833.SRP035479.SRX435693 RNASeq.elegans.WBStrain00000001.WBls:0000038.Hermaphrodite.WBbt:0007833.SRP035479.SRX435694 RNASeq.elegans.WBStrain00035559.WBls:0000038.Hermaphrodite.WBbt:0007833.SRP035479.SRX435695 RNASeq.elegans.WBStrain00035559.WBls:0000038.Hermaphrodite.WBbt:0007833.SRP035479.SRX435696 RNASeq.elegans.WBStrain00035559.WBls:0000038.Hermaphrodite.WBbt:0007833.SRP035479.SRX435697 RNASeq.elegans.WBStrain00035559.WBls:0000038.Hermaphrodite.WBbt:0007833.SRP035479.SRX435698 RNASeq.elegans.WBStrain00035559.WBls:0000038.Hermaphrodite.WBbt:0007833.SRP035479.SRX435699 RNASeq.elegans.WBStrain00035559.WBls:0000038.Hermaphrodite.WBbt:0007833.SRP035479.SRX435700.
Tags	Method: RNAseq, Species: Caenorhabditis elegans, Topic: aging, Topic: disease, Topic: response to chemical