SPELL - Nematode

Citation	Schwarz EM, Hu Y, Antoshechkin I, Miller MM, Sternberg PW, Aroian RV. The genome and transcriptome of the zoonotic hookworm Ancylostoma ceylanicum identify infection-specific gene families. Nat Genet, 2015.
PubMed ID	25730766
Short Description	The genome and transcriptome of the zoonotic hookworm Ancylostoma ceylanicum identify infection-specific gene families. GEO Record: N.A. Platform: N.A. Download gene-centric, log2 transformed data: WBPaper00046511.ce.rs.csv
# of Conditions	2
Full Description	Hookworms infect over 400 million people, stunting and impoverishing them. Sequencing hookworm genomes and finding which genes they express during infection should help in devising new drugs or vaccines against hookworms. Unlike other hookworms, Ancylostoma ceylanicum infects both humans and other mammals, providing a laboratory model for hookworm disease. We determined an A. ceylanicum genome sequence of 313 Mb, with transcriptomic data throughout infection showing expression of 30,738 genes. Approximately 900 genes were upregulated during early infection in vivo, including ASPRs, a cryptic subfamily of activation-associated secreted proteins (ASPs). Genes downregulated during early infection included ion channels and G protein-coupled receptors; this downregulation was observed in both parasitic and free-living nematodes. Later, at the onset of heavy blood feeding, C-lectin genes were upregulated along with genes for secreted clade V proteins (SCVPs), encoding a previously undescribed protein family. These findings provide new drug and vaccine targets and should help elucidate hookworm pathogenesis. Experimental Details: RNASeq.elegans.WBStrain00000001.WBls:0000038.Hermaphrodite.WBbt:0003810.SRP035507.SRX437635 RNASeq.elegans.WBStrain00000001.WBls:0000038.Hermaphrodite.WBbt:0003810.SRP035507.SRX437640.
Tags	Method: RNAseq, Species: Caenorhabditis elegans, Topic: response to chemical