| Citation | Arndts K, Specht S, Debrah AY, Tamarozzi F, Klarmann Schulz U, Mand S, Batsa L, Kwarteng A, Taylor M, Adjei O, Martin C, Layland LE, Hoerauf A. Immunoepidemiological profiling of onchocerciasis patients reveals associations with microfilaria loads and ivermectin intake on both individual and community levels. PLoS Negl Trop Dis, 2014. |
| PubMed ID | 24587458 |
| Short Description | Immunoepidemiological profiling of onchocerciasis patients reveals associations with microfilaria loads and ivermectin intake on both individual and community levels. GEO Record: N.A. Platform: N.A. Download gene-centric, log2 transformed data: WBPaper00048621.ovo.rs.csv |
| # of Conditions | 9 |
Full Description
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Mass drug administration (MDA) programmes against Onchocerca volvulus use ivermectin (IVM) which targets microfilariae (MF), the worm's offspring. Most infected individuals are hyporesponsive and present regulated immune responses despite high parasite burden. Recently, with MDA programmes, the existence of amicrofilaridermic (a-MF) individuals has become apparent but little is known about their immune responses. Within this immunoepidemiological study, we compared parasitology, pathology and immune profiles in infection-free volunteers and infected individuals that were MF(+) or a-MF. The latter stemmed from villages in either Central or Ashanti regions of Ghana which, at the time of the study, had received up to eight or only one round of MDA respectively. Interestingly, a-MF patients had fewer nodules and decreased IL-10 responses to all tested stimuli. On the other hand, this patient group displayed contrary IL-5 profiles following in vitro stimulation or in plasma and the dampened response in the latter correlated to reduced eosinophils and associated factors but elevated neutrophils. Furthermore, multivariable regression analysis with covariates MF, IVM or the region (Central vs. Ashanti) revealed that immune responses were associated with different covariates: whereas O. volvulus-specific IL-5 responses were primarily associated with MF, IL-10 secretion had a negative correlation with times of individual IVM therapy (IIT). All plasma parameters (eosinophil cationic protein, IL-5, eosinophils and neutrophils) were highly associated with MF. With regards to IL-17 secretion, although no differences were observed between the groups to filarial-specific or bystander stimuli, these responses were highly associated with the region. These data indicate that immune responses are affected by both, IIT and the rounds of IVM MDA within the community. Consequently, it appears that a lowered infection pressure due to IVM MDA may affect the immune profile of community members even if they have not regularly participated in the programmes. Experimental Details: RNASeq.ovolvulus.WBStrain00041977.WBls:0000104.Female.WBbt:0007833.SRP056861.SRX978156 RNASeq.ovolvulus.WBStrain00041977.WBls:0000104.Female.WBbt:0007833.SRP056861.SRX978157 RNASeq.ovolvulus.WBStrain00041977.WBls:0000104.Female.WBbt:0007833.SRP056861.SRX978158 RNASeq.ovolvulus.WBStrain00041977.WBls:0000104.Female.WBbt:0007833.SRP056861.SRX978159 RNASeq.ovolvulus.WBStrain00041977.WBls:0000104.Female.WBbt:0007833.SRP056861.SRX978160 RNASeq.ovolvulus.WBStrain00041977.WBls:0000104.Female.WBbt:0007833.SRP056861.SRX978161 RNASeq.ovolvulus.WBStrain00041977.WBls:0000104.Male.WBbt:0007833.SRP056861.SRX978162 RNASeq.ovolvulus.WBStrain00041977.WBls:0000104.Female.WBbt:0007833.SRP056861.SRX978163 RNASeq.ovolvulus.WBStrain00041977.WBls:0000104.Female.WBbt:0007833.SRP056861.SRX978164. |
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