SPELL - Nematode

Citation	Yanai I, Baugh LR, Smith JJ, Roehrig C, Shen-Orr SS, Claggett JM, Hill AA, Slonim DK, Hunter CP. Pairing of competitive and topologically distinct regulatory modules enhances patterned gene expression. Mol Syst Biol, 2008.
PubMed ID	18277379
Short Description	Pairing of competitive and topologically distinct regulatory modules enhances patterned gene expression. GEO Record: GSE9665 Platform: GPL200 Download gene-centric, log2 transformed data: WBPaper00031525.ce.mr.csv
# of Conditions	74
Full Description	Biological networks are inherently modular, yet little is known about how modules are assembled to enable coordinated and complex functions. We used RNAi and time series, whole-genome microarray analyses to systematically perturb and characterize components of a Caenorhabditis elegans lineage-specific transcriptional regulatory network. These data are supported by selected reporter gene analyses and comprehensive yeast one-hybrid and promoter sequence analyses. Based on these results, we define and characterize two modules composed of muscle- and epidermal-specifying transcription factors that function together within a single cell lineage to robustly specify multiple cell types. The expression of these two modules, although positively regulated by a common factor, is reliably segregated among daughter cells. Our analyses indicate that these modules repress each other, and we propose that this cross-inhibition coupled with their relative time of induction function to enhance the initial asymmetry in their expression patterns, thus leading to the observed invariant gene expression patterns and cell lineage. The coupling of asynchronous and topologically distinct modules may be a general principle of module assembly that functions to potentiate genetic switches. Experimental Details: WBPaper00031525:mex-3_143_A WBPaper00031525:mex-3_143_B WBPaper00031525:mex-3_186_A WBPaper00031525:mex-3_186_B WBPaper00031525:mex-3_230_A WBPaper00031525:mex-3_230_B WBPaper00031525:mex-3_scrt-1_186_A WBPaper00031525:mex-3_tbx-8_tbx-9_143_A WBPaper00031525:mex-3_tbx-8_tbx-9_143_B WBPaper00031525:mex-3_tbx-8_tbx-9_143_C WBPaper00031525:mex-3_tbx-8_tbx-9_186_A WBPaper00031525:mex-3_elt-1_143_A WBPaper00031525:mex-3_elt-1_143_B WBPaper00031525:mex-3_elt-1_143_C WBPaper00031525:mex-3_elt-1_186_A WBPaper00031525:mex-3_elt-1_186_B WBPaper00031525:mex-3_elt-1_186_C WBPaper00031525:mex-3_vab-7_186_A WBPaper00031525:mex-3_vab-7_230_A WBPaper00031525:mex-3_vab-7_230_B WBPaper00031525:mex-3_vab-7_230_C WBPaper00031525:mex-3_hlh-1_186_A WBPaper00031525:mex-3_hlh-1_186_B WBPaper00031525:mex-3_hlh-1_230_A WBPaper00031525:mex-3_nhr-25_186_A WBPaper00031525:mex-3_nhr-25_186_B WBPaper00031525:mex-3_nhr-25_186_C WBPaper00031525:mex-3_nhr-25_230_A WBPaper00031525:mex-3_nhr-25_230_B WBPaper00031525:mex-3_elt-3_186_A WBPaper00031525:mex-3_elt-3_230_A WBPaper00031525:mex-3_elt-3_230_B WBPaper00031525:mex-3_elt-3_230_C WBPaper00031525:mex-3_nob-1_186_A WBPaper00031525:mex-3_nob-1_186_B WBPaper00031525:mex-3_nob-1_186_C WBPaper00031525:mex-3_nob-1_230_A WBPaper00031525:mex-3_nob-1_230_B WBPaper00031525:mex-3_nob-1_230_C WBPaper00031525:mex-3_unc-120_186_A WBPaper00031525:mex-3_unc-120_186_B WBPaper00031525:mex-3_unc-120_186_C WBPaper00031525:mex-3_unc-120_230_A WBPaper00031525:mex-3_unc-120_230_B WBPaper00031525:mex-3_unc-120_230_C WBPaper00031525:mex-3_hnd-1_143_A WBPaper00031525:mex-3_hnd-1_143_B WBPaper00031525:mex-3_hnd-1_143_C WBPaper00031525:mex-3_hnd-1_186_A WBPaper00031525:mex-3_hnd-1_186_B WBPaper00031525:mex-3_lin-26_186_A WBPaper00031525:mex-3_lin-26_186_B WBPaper00031525:mex-3_lin-26_230_A WBPaper00031525:mex-3_lin-26_230_B WBPaper00031525:mex-3_scrt-1_143_A WBPaper00031525:mex-3_scrt-1_143_B WBPaper00031525:mex-3_scrt-1_186_B WBPaper00031525:mex-3_pal-1_143_A WBPaper00031525:mex-3_scrt-1_186_C WBPaper00031525:mex-3_vab-7_186_B WBPaper00031525:mex-3_vab-7_186_C WBPaper00031525:mex-3_hlh-1_230_B WBPaper00031525:mex-3_nhr-25_230_C WBPaper00031525:mex-3_elt-3_186_B WBPaper00031525:mex-3_elt-3_186_C WBPaper00031525:mex-3_hnd-1_186_C WBPaper00031525:mex-3_pal-1_101_A WBPaper00031525:mex-3_pal-1_143_B WBPaper00031525:mex-3_pal-1_143_C WBPaper00031525:mex-3_pal-1_186_A WBPaper00031525:mex-3_pal-1_186_B WBPaper00031525:mex-3_pal-1_186_C WBPaper00031525:mex-3_pal-1_230_A WBPaper00031525:mex-3_pal-1_230_B.
Tags	Method: microarray, Species: Caenorhabditis elegans, Topic: RNAi gene function study