| Citation | Dawe AS, Bodhicharla RK, Graham NS, May ST, Reader T, Loader B, Gregory A, Swicord M, Bit-Babik G, de Pomerai DI. Low-intensity microwave irradiation does not substantially alter gene expression in late larval and adult Caenorhabditis elegans. Bioelectromagnetics, 2009. |
| PubMed ID | 19533680 |
| Short Description | Low-intensity microwave irradiation does not substantially alter gene expression in late larval and adult Caenorhabditis elegans. GEO Record: GSE10787 Platform: GPL200 Download gene-centric, log2 transformed data: WBPaper00034636.ce.mr.csv |
| # of Conditions | 14 |
Full Description
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Reports that low-intensity microwave radiation induces heat-shock reporter gene expression in the nematode, Caenorhabditis elegans, have recently been reinterpreted as a subtle thermal effect caused by slight heating. This study used a microwave exposure system (1.0 GHz, 0.5 W power input; SAR 0.9-3 mW kg(-1) for 6-well plates) that minimises temperature differentials between sham and exposed conditions (< or =0.1 degrees C). Parallel measurement and simulation studies of SAR distribution within this exposure system are presented. We compared five Affymetrix gene arrays of pooled triplicate RNA populations from sham-exposed L4/adult worms against five gene arrays of pooled RNA from microwave-exposed worms (taken from the same source population in each run). No genes showed consistent expression changes across all five comparisons, and all expression changes appeared modest after normalisation (< or =40% up- or down-regulated). The number of statistically significant differences in gene expression (846) was less than the false-positive rate expected by chance (1131). We conclude that the pattern of gene expression in L4/adult C. elegans is substantially unaffected by low-intensity microwave radiation; the minor changes observed in this study could well be false positives. As a positive control, we compared RNA samples from N2 worms subjected to a mild heat-shock treatment (30 degrees C) against controls at 26 degrees C (two gene arrays per condition). As expected, heat-shock genes are strongly up-regulated at 30 degrees C, particularly an hsp-70 family member (C12C8.1) and hsp-16.2. Under these heat-shock conditions, we confirmed that an hsp-16.2::GFP transgene was strongly up-regulated, whereas two non-heat-inducible transgenes (daf-16::GFP; cyp-34A9::GFP) showed little change in expression. Experimental Details: WBPaper00034636:Sham__1_S1 WBPaper00034636:Microwave__2_E1 WBPaper00034636:Sham__3_S2 WBPaper00034636:Microwave__4_E2 WBPaper00034636:Sham__5_S3 WBPaper00034636:Microwave__6_E3 WBPaper00034636:Sham__7_S4 WBPaper00034636:Microwave__8_E4 WBPaper00034636:Sham__9_S5 WBPaper00034636:Microwave__10_E5 WBPaper00034636:heat_control__1 WBPaper00034636:heat_control__2 WBPaper00034636:mild_heat_shock__1 WBPaper00034636:mild_heat_shock__2. |
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