| Citation | Boyd WA, Crocker TL, Rodriguez AM, Leung MC, Lehmann DW, Freedman JH, Van Houten B, Meyer JN. Nucleotide excision repair genes are expressed at low levels and are not detectably inducible in Caenorhabditis elegans somatic tissues, but their function is required for normal adult life after UVC exposure. Mutat Res, 2010. |
| PubMed ID | 19879883 |
| Short Description | Nucleotide excision repair genes are expressed at low levels and are not detectably inducible in Caenorhabditis elegans somatic tissues, but their function is required for normal adult life after UVC exposure. GEO Record: GSE15159 Platform: GPL200 Download gene-centric, log2 transformed data: WBPaper00035429.ce.mr.csv |
| # of Conditions | 24 |
Full Description
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We performed experiments to characterize the inducibility of nucleotide excision repair (NER) in Caenorhabditis elegans, and to examine global gene expression in NER-deficient and -proficient strains as well as germline vs. somatic tissues, with and without genotoxic stress. We also carried out experiments to elucidate the importance of NER in the adult life of C. elegans under genotoxin-stressed and control conditions. Adult lifespan was not detectably different between wild-type and NER-deficient xpa-1 nematodes under control conditions. However, exposure to 6J/m(2)/day of ultraviolet C radiation (UVC) decreased lifespan in xpa-1 nematodes more than a dose of 100 J/m(2)/day in wild-type. Similar differential sensitivities were observed for adult size and feeding. Remarkably, global gene expression was nearly identical in young adult wild-type and xpa-1 nematodes, both in control conditions and 3h after exposure to 50 J/m(2) UVC. Neither NER genes nor repair activity were detectably inducible in young adults that lacked germ cells and developing embryos (glp-1 strain). However, expression levels of dozens of NER and other DNA damage response genes were much (5-30-fold) lower in adults lacking germ cells and developing embryos, suggesting that somatic and post-mitotic cells have a much lower DNA repair ability. Finally, we describe a refinement of our DNA damage assay that allows damage measurement in single nematodes. Experimental Details: WBPaper00035429:glp-1_UV_2008_rep1 WBPaper00035429:glp-1_UV_2008_rep2 WBPaper00035429:N2_control_2007_rep2 WBPaper00035429:N2_control_2007_rep1 WBPaper00035429:N2_UV_2007_rep2 WBPaper00035429:N2_UV_2007_rep1 WBPaper00035429:glp-1_control_2007_rep1 WBPaper00035429:glp-1_control_2007_rep2 WBPaper00035429:glp-1_UV_2007_rep1 WBPaper00035429:glp-1_UV_2007_rep2 WBPaper00035429:xpa-1_control_2007_rep1 WBPaper00035429:xpa-1_control_2007_rep2 WBPaper00035429:xpa-1_UV_2007_rep2 WBPaper00035429:xpa-1_UV_2007_rep1 WBPaper00035429:xpa-1_control_2008_rep1 WBPaper00035429:xpa-1_control_2008_rep2 WBPaper00035429:xpa-1_UV_2008_rep1 WBPaper00035429:xpa-1_UV_2008_rep2 WBPaper00035429:N2_control_2008_rep1 WBPaper00035429:N2_control_2008_rep2 WBPaper00035429:N2_UV_2008_rep2 WBPaper00035429:N2_UV_2008_rep1 WBPaper00035429:glp-1_control_2008_rep1 WBPaper00035429:glp-1_control_2008_rep2. |
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