| Citation | Zisoulis DG, Lovci MT, Wilbert ML, Hutt KR, Liang TY, Pasquinelli AE, Yeo GW. Comprehensive discovery of endogenous Argonaute binding sites in Caenorhabditis elegans. Nat Struct Mol Biol, 2010. |
| PubMed ID | 20062054 |
| Short Description | Comprehensive discovery of endogenous Argonaute binding sites in Caenorhabditis elegans. GEO Record: GSE19138 Platform: GPL200 Download gene-centric, log2 transformed data: WBPaper00035588.ce.mr.csv |
| # of Conditions | 6 |
Full Description
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MicroRNAs (miRNAs) regulate gene expression by guiding Argonaute proteins to specific target mRNA sequences. Identification of bona fide miRNA target sites in animals is challenging because of uncertainties regarding the base-pairing requirements between miRNA and target as well as the location of functional binding sites within mRNAs. Here we present the results of a comprehensive strategy aimed at isolating endogenous mRNA target sequences bound by the Argonaute protein ALG-1 in C. elegans. Using cross-linking and ALG-1 immunoprecipitation coupled with high-throughput sequencing (CLIP-seq), we identified extensive ALG-1 interactions with specific 3' untranslated region (UTR) and coding exon sequences and discovered features that distinguish miRNA complex binding sites in 3' UTRs from those in other genic regions. Furthermore, our analyses revealed a striking enrichment of Argonaute binding sites in genes important for miRNA function, suggesting an autoregulatory role that may confer robustness to the miRNA pathway. Experimental Details: WBPaper00035588:ALG-1(-)_1 WBPaper00035588:ALG-1(-)_2 WBPaper00035588:ALG-1(-)_3 WBPaper00035588:WT_1 WBPaper00035588:WT_2 WBPaper00035588:WT_3. |
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