SPELL - Nematode

Citation	Skjeldam HK, Kassahun H, Fensgard O, SenGupta T, Babaie E, Lindvall JM, Arczewska K, Nilsen H. Loss of Caenorhabditis elegans UNG-1 uracil-DNA glycosylase affects apoptosis in response to DNA damaging agents. DNA Repair (Amst), 2010.
PubMed ID	20493785
Short Description	Loss of Caenorhabditis elegans UNG-1 uracil-DNA glycosylase affects apoptosis in response to DNA damaging agents. GEO Record: GSE21467 Platform: GPL200 Download gene-centric, log2 transformed data: WBPaper00036291.ce.mr.csv
# of Conditions	6
Full Description	The nematode Caenorhabditis elegans has been used extensively to study responses to DNA damage. In contrast, little is known about DNA repair in this organism. C. elegans is unusual in that it encodes few DNA glycosylases and the uracil-DNA glycosylase (UDG) encoded by the ung-1 gene is the only known UDG. C. elegans could therefore become a valuable model organism for studies of the genetic interaction networks involving base excision repair (BER). As a first step towards characterization of BER in C. elegans, we show that the UNG-1 protein is an active uracil-DNA glycosylase. We demonstrate that an ung-1 mutant has reduced ability to repair uracil-containing DNA but that an alternative Ugi-inhibited activity is present in ung-1 nuclear extracts. Finally, we demonstrate that ung-1 mutants show altered levels of apoptotic cell corpses formed in response to DNA damaging agents. Increased apoptosis in the ung-1 mutant in response to ionizing radiation (IR) suggests that UNG-1 contributes to repair of IR-induced DNA base damage in vivo. Following treatment with paraquat however, the apoptotic corpse-formation was reduced. Gene expression profiling suggests that this phenotype is a consequence of compensatory transcriptomic shifts that modulate oxidative stress responses in the mutant and not an effect of reduced DNA damage signaling. Experimental Details: WBPaper00036291:ung-1_bio_rep1 WBPaper00036291:ung-1_bio_rep2 WBPaper00036291:ung-1_bio_rep3 WBPaper00036291:N2_bio_rep1 WBPaper00036291:N2_bio_rep2 WBPaper00036291:N2_bio_rep3.
Tags	Method: microarray, Species: Caenorhabditis elegans, Topic: programmed cell death